TABLE 5

Phenotypes of multicopy suppressors in pseudohyphal-deficient strains

Pseudohyphal phenotype when expressed in:
GeneΔmep1 Δmep2wild-typeΔmep2Δgpa2Δgpa2 Δmep1Δste11
None-+-±±-
MEP2+++±±-
PHD1±++±±++
TEC1+++±++±
PHD2±+++±++
MSS11±++±±+±
MGA1+++++±++±
SKN7±++±±++
MSN5±++±±±±
CDC6+++±+++
HMS1+++±±++
HMS2±++±±++++
DOT6±+±±±±
MEP1±+-±±-
MEP3±+-±±-
URE2±+-±±±
DAL80±+±±±±
SRK1±+-±±±
  • Library plasmids with each of the above genes were used to transform wild-type (MLY61), Δmep1/Δmep1 Δmep2/Δmep2 (MLY115a/α), Δmep2/Δmep2 (MLY108a/α), Δgpa2/Δgpa2 (MLY132a/α), Δgpa2/Δgpa2 Δmep1/Δmep1 (MLY135a/α), or Δstel1/Δstel1 (HLY351) diploid strains, and were incubated for 4 days at 30°.