TABLE 4

Nitrogen regulation of filamentous growth induced by expression of 2μ, suppressors

Pseudohyphal formation
(NH4)2SO4
Gene50 μm500 μm5 mm100 μm Gln
None+±-+
MEP2+±-+
PHD1+++-++
TEC1++±-++
PHD2++±-++
MSS11++±-++
MGA1++++-++
SkN7++±-++
MSN5++±-+
CDC6+++-++
HMS1+++-++
HMS2+±-+
DOT6+±-+
  • Wild-type diploid (MLY61) strains expressing each of the genes listed above were incubated on the indicated media for 2 days [500 (μm and 5 mm (NH4)2SO4] or 4 days [50 (μm (NH4)2SO4 and 100 (μm glutamine] at 30°.