TABLE 4

Repression of hypoxic genes is unaffected by H3 and H4 terminal deletions

GeneaStrainbDerepressedcRepressedFold repressiond
ANB1WT1872.381
H3ΔN1341.874
H4ΔN1202.060
COX5BWT1.60.82.0
H3ΔN1.50.72.1
H4ΔN2.90.93.2
AAC3WT3.80.49.5
H3ΔN1.70.44.2
H4ΔN1.70.35.7
SUC2WT232.59.2
H3ΔN164.73.4
H4ΔN197.22.6
  • a β-Galactosidase assays were carried on permeabilized cells transformed with the ANB1 (YCp(33)AZ), COX5B, or AAC3 lacZ fusions described in materials and methods. Invertase assays were carried out to determine levels of expression of the genomic SUC2 gene.

  • b The strain P1/I8 was used carrying a plasmid with a wild-type H3 and H4 gene (WT) or a plasmid with either the H3 N-terminal deletion and wild-type H4 gene (H3ΔN) or the H4 N-terminal deletion and the wild-type H3 gene (H4ΔN) as described in materials and methods.

  • c Cells carrying the ANB1,COX5B, and AAC3 lacZ fusions were grown on selective medium either anaerobically (derepressed) or aerobically (repressed). For invertase assays, cells were grown in YP medium containing either 2% raffinose (derepressed) or 4% glucose (repressed).

  • d Fold repression was determined by dividing the derepressed value by the repressed value.