TABLE 3

Effect of Rox1-bindging site mutations on repression of ANB1 expression

β-Galactosidase
activity
Fold repression
rox1/wild type)
ANB1 mutationWild typeΔrox1
ΔOpB1.511476
OpA(A3)ΔOpB1012112
OpA(2A3)ΔOpB21944.5
OpA(G3)ΔOpB3.611833
OpA(A9)ΔOpB28802.8
  • β-Galactosidase assays were carried out in permeabilized cells of strain RZ53-6 (wild type) or RZ53-6Arox1 transformed with the indicated plasmids. Cells were grown aerobically in SC-uracil medium to select for the plasmid.