TABLE 1

Rox1-binding sites in hypoxic regulatory regions

GeneOperatoraEvidencebReferences
HEM13-584 CGT ATTCTT CAA -595Deletion analysisAmillet et al. (1995)
-475 TCA ATTGTT TAG -464
-238 TGC TTTGTT CAA -249
-185 CCC ATTGTT CTC -174
ERG11-429 CTT ATTGTC TTC -440ConsensusTuri and Loper (1982)
-358 CCT ATTGTG CAT -247
HMG2- 282 CGC ATTGTT TTG - 271ConsensusThorsness et al. (1989); Yeast Genome Database
CPR1-95 TCA TTTGTT CCT -84ConsensusTuri and Loper (1992)
SUT1-361 CGT ATTGTT GAA -372ConsensusBouret and Karst (1995)
-342 AGC TTTGTT CTT -331
-243 GTT TTTGTT CTT -232
-115 GTT ATTGTT TCA - 104
OLE1-272 CCT ATTGTT ACG -261ConsensusStukey et al. (1990)
-212 TAT ATTGTT AAC -223
COX5b-367 TGA ATTGTT TTA - 378ConsensusHodge et al. (1990)
- 228 TGT ATTGTT CGA -217
CYC7-499 GTA ATTGTC TCC -488ConsensusZitomer et al. (1987)
-333 CCT ATTGTA TTA -322
AAC3-197 TTC ATTGTT TGG -186Deletion analysisSabova et al. (1993)
-145 TCC ATTGTT CTT -134
ANB1- 316 TCC ATTGTT CGT -305Deletion analysisLowry et al. (1990);
- 285 CCT ATTGTT CTC -274    gel retardationBalasubramanian et al. (1993)
- 218 TCC ATTGTT CTC -207
- 197 CTC ATTGTT GCT -186
ROX1- 397 CCT ATTGTT GCT -386Deletion analysis,Deckert et al. (1995a)
- 364 CGT ATTGTC TTG -353    gel retardation
ConsensusYYY ATTGTT CTCc
  • The table was adapted from Zitomer et al. (1997a). A search for Rox1-binding sites in the upstream regions of hypoxic genes yielded the sequences presented in the table. The significance of several of the putative Rox1 operators has been established by deletion analysis or gel retardation experiments as indicated. Comparison of the sequences resulted in the consensus sequence identified previously (Lowry et al. 1990).

  • a Sequences are numbered with the first base in the coding strand immediately 5′ to the ATG initiation codon as -1.

  • b Consensus indicates the operators were identified solely by sequence homology.

  • c Y indicates pyrimidine.