TABLE 4

A Ste5p RING-H2 domain mutation abolishes the interaction with Ste4p

GADSte4
Ste52.0 ± 0.30120 ± 7.1
Ste5 C177S0.30 ± 0.0700.40 ± 0.020
  • Quantitative β-galactosidase assays were carried out in strain M364-2C for the interaction of GAD-Ste4 with either LexA-Ste5 or LexA-Ste5 C177S. Numbers are the average of two samples, in arbitrary units.