TABLE 3

The Ste4 L65S-Ste5 interaction is temperature sensitive

GADSte4L49PL65S
Ste18 20°5.8 ± 1.0590 ± 20390 ± 9.9480 ± 53
Ste18 30°0.0 ± 0.0450 ± 2.2150 ± 10230 ± 41
Akr1 20°0.0 ± 0.0170 ± 8.70.0 ± 0.0200 ± 3.6
Akr1 30°0.90 ± 0.25280 ± 374.5 ± 0.0360 ± 100
Ste5 20°0.0 ± 0.0200 ±100.0 ± 0.0180 ± 45
Ste5 30°0.10 ± 0.20250 ± 571.7 ± 0.313.5 ± 2.2
  • Quantitative β-galactosidase assays for two-hybrid interactions of GAD-Ste4 or GAD-Ste4 L49P and L65S mutants with LexA-Ste18, lexA-Akr1, and LexA-Ste5. The transformants (of strain M364-2C) were grown with shaking in liquid culture for 48 hr, at the temperatures indicated, then assayed for β-galactosidase activity. Numbers given are the average of three samples in arbitrary units.