TABLE 2

Interaction defects of Ste4p mutants

GADSte4D224AL49PL65SD62N
LexA0.0 ± 0.00.0 ± 0.00.0 ± 0.00.0 ± 0.00.37 ± 0.00500.98 ± 0.52
Gpa10.48 ± 0.23260 ± 0.854.7 ± 1.4330 ± 46240 ± 21130 ± 33
Ste181.4 ± 1.4400 ± 93370 ± 7.184 ± 20110 ± 4.6280 ± 19
Ste51.9 ± 0.20350 ± 43150 ± 181.1 ± 0.512.8 ± 0.12160 ± 31
Akr10.0 ± 0.094 ± 1.484 ± 3.00.14 ± 0.1444 ± 10160 ± 5.0
  • Quantitative β-galactosidase assays were carried out for two-hybrid interactions of GAD-Ste4 or GAD-Ste4 mutants in combination with the LexA fusions as indicated, in strain M364-2C. Numbers given are the average of three samples in arbitrary units.