TABLE 1

Yeast strains

StrainGenotypea and phenotypeSource
21RMATa ura3-52 leu2-3,112 ade1Rowley et al. (1991)
68507AMATα cdc68-1 ura3-52 Ade-Rowley et al. (1991)
ART68-1MATa cdc68-1 ura3-52 leu2-3,112 ade2Rowley et al. (1991)
L577MATα cdc68-197his4-912∂ lys2-128∂ ura3-52Malone et al. (1991)
LY60MATα cdc68-11 HO::lacZ46 ura3 leu2-3,112 trp1-1 can1-100 Ade- Met-Lycan et al. (1994)
FY56MATα his4-912∂ lys2-128∂ ura3-52Malone et al. (1991)
BM64MATa/MATα cdc68-101::LEU2/+ his4-912∂/his4-912∂ lys2-128∂/
    lys2-128∂ ura3-52/ura3-52 leu2-3,112/leu2-3,112 trp1-1/trp1-1
Malone et al. (1991)
DE13aMATa cdc68-101::LEU2 his4-912∂ lys2-128∂ ura3-52 leu2-3,112
    trp1-1 pBM13(TRP1 CDC68)
BM64 segregant har-
    boring pBM13
DE4BBM64 with cdc68-101::LEU2 replaced by cdc68-K922This study
DE4B-17aMATα his4-912∂ lys2-128∂ ura3-52 leu2-3, 112 trp1-1DE4B segregant
DE4B-17bMATa cdc68-Δ922 his4-912∂ lys2-128∂ ura3-52 leu2-3, 112 trp1-1DE4B segregant
DE4B-17cMATα cdc68-Δ922 his4-912∂ lys2-128∂ ura3-52 leu2-3, 112 trp1-1DE4B segregant
FY711MATα snf5::UTA3 his4-912∂ lys2-128∂ ura3-52 leu2-Δ1F. Winston
BM404MATa suc2ΔUAS(-1900/-390) his4-912∂ lys2-128∂ ura3-52E. A. Malone
BM403MATa cdc68-197 suc2ΔUAS(-1900/-390) his4-912∂ lys2-128∂ ura3-52E. A. Malone
ARI68-7MATa cdc68-1[URA3] ura3-52 leu2-3,112 ade2Rowley et al. (1991)
QX3MATα cdc68-1 san1-3 ura3-52 ade2Xu et al. (1993)
DE68S-2dMATα cdc68-1[URA3] san1-3 ura3-52 leu2-3,112 ade2ARI68-7 X QX3
    segregant
DE81-2bMATa cdc68-Δ922 san1-3 his4-912∂ lys2-128∂ trp1-1 Ade-DE68S-2d X DE4B-
    17b segregant
DE81-6cMATα cdc68-Δ922 san1-3 lys2-128∂ ura3-52 leu2-3,112DE68S-2d X DE4B-
    17b segregant
QXN1MATa san1Δ::URA3 ura3-52 leu2-3,112 Ade-Xu et al. (1993)
DE1N-15dMAT? cdc68-Δ922 san1Δ::URA3 lys2-128∂ ura3-52QXN1 X DE81-6c
    segregant
  • a Square brackets enclose plasmid-derived sequences integrated in single copy. cdc68-Δ922 genotype confirmed by Southern analysis.