TABLE 4

MIG1 and MIG2 expression

Mean β-galactosidase activity (Miller units ± SD)a
MIG1-LacZMIG2-LacZ
Relevant genotypeRDD/RRDD/R
WT27 ± 8325 ± 51122478 ± 4413969 ± 3571.6
mig1Δ144 ± 38631 ± 1294NDND
mig2Δ64 ±11342 ± 965NDND
yer028Δ71 ± 14365 ± 495NDND
mig1Δ mig2Δ400 ±113535 ± 761.3NDND
mig2Δ yer028Δ85 ± 5296 ± 453.5NDND
mig1Δ mig2Δ yer028Δ547 ± 87824 ± 1341.52688 ± 2942268 ± 2100.8
  • Strains: WT, YM4797; mig1Δ, YM4966; mig2Δ, YM4664; yer028Δ, YM4734; mig1Δmig2Δ, YM4738; mig2Δyer028Δ, YM4742; mig1Δ mig2Δ yer028Δ, YM4847. Plasmids: pBM3190, pBM3295.

  • a Cells were grown in synthetic media lacking only uracil and containing either 4% glucose (R), or 5% glycerol and 0.05% glucose (D). The values are Miller units of β-galactosidase activity averaged from two to three independent tranformants assayed in duplicate. D/R, fold depression; ND, not done.