TABLE 7

Phenotypic analysis of suppressor deletion strains

Invasive growthCell morphologyBudding pattern
GenotypeFilamentous growthHaploidDiploid% YF% PH% Bipolar% Axial/unipolar% Random
Wildtype++++++87.212.859.327.813.0
Δmep2-+++97.12.951.826.421.8
Δmep1 Δmep2-++86.113.952.024.024.0
Δtec1--+97.12.954.424.820.8
Δphd2-±-75.724.325.225.249.6
Δmss11---100<144.216.838.9
Δmga1±±++92.27.841.517.840.7
Δmsn5±++++86.113.973.225.019.6
Δure2-±+100<146.711.741.7
Δphd1+++++97.12.964.419.216.3
Δskn7+±++86.813.2NDaNDND
Δhms1+++++78.421.656.223.820.0
Δhms2++++83.416.658.220.920.9
Δdot6+±+++96.04.055.929.414.7
Δdal80+++++96.63.458.230.011.8
  • Strains of the indicated genotype were analysed for invasive growth, cellular morphology, and budding pattern as described in materials and methods. PH, pseudohyphal form; YF, yeast form.

  • a Δskn7/Δskn7 mutant strains form aggregates with diffuse staining with Calcofluor, obscuring the budding pattern.