TABLE 6

Suppressor deletion strains: phenotypes and epistasis with previously characterized alleles

StrainGenotypeVectorRAS2Val19GPA2Val132STE11-4STE12PHD1TEC1
MLY61Wild type++++++++++++
MLY132a/αΔgpa2±++++±±±±
MLY108a/αΔmep2-++±+±+
MLY115a/αΔmep1 Δmep2-++++++
MLY183a/αΔtec1--±---+
MLY180a/αΔphd2-±+±±++±
MLY181a/αΔmss11-±+a±+a±±
MLY179a/αΔmga1±+++a++a+++
MLY170a/αΔmsn5±++++++++
MLY182a/αΔphd1+
MLY172a/αΔskn7+
MLY168a/αΔhms1+
MLY169a/αΔhms2+
MLY191a/αΔdot6+
MLY224a/αΔdal80+
  • Homozygous diploid strains of the indicated genotypes were incubated on SLAD medium (SLARG medium to induce GPA2Val132 and SLADG medium to induce pGAL-STE12) for 4 days (6 days for GPA2Val132 and pGAL-STE12).

  • a Δmga1/Δmga1 and Δmss11/Δmss11 strains filament on media containing galactose as the carbon source. As a result, the suppression by the indicated alleles is probably an artifact of the carbon source.