TABLE 3

Disruption of silencing by DOT genes

OverexpressionDeletion
GeneTelomeresHMrDNATelomeresHMrDNA
DOT1 (YDR440W; PCH1)++++++++++b+++ +++ NC
TLC1a ++++NCNCNCNCNC
DNA2 (YHR164C)+++++++NANANA
DOT4 (YNL186W)+++++++++ + c+ + c +c
DOT5 (YIL010W)++++NCNCNCNC
DOT6 (YER088C)++++++NCNCNC
IFH1 (YLR223C)+++++++NANANA
ASF1 (YJL115W)++++++++++++
RPL32 (YBL092W)++NCNANANA
SIR4 (YDR227W)++++++++++++++++

A summary of the silencing phenotypes of the DOT genes at telomeres, HM loci, and rDNA.

+, increase in URA3 expression caused by the disruption of silencing;

–, a decrease in URA3 expression caused by an enhancement of silencing; NC, no change; NA, not applicable because the gene is essential for viability.

  • a TLC1 is located on the Watson strand between YBR035C and YBR036C.

  • b Increased FOA sensitivity, but no change in growth on media lacking uracil.

  • c dot4Δ strains show an increase in resistance to 6-AU.