TABLE 9

Maternal rescue of developmental arrest

ParentTemperature% L4% Dauer larvaeaOtherbNc
m41/+25.5°87 ± 912 ± 8 (48)0.6 ± 2302 (8)
e979/+25.5°75 ± 425 ± 4 (100)d0457 (8)
m65/+25.5°75 ± 323 ± 3 (92)1.8 ± 0.51361 (6)
+ / qC125.5°97 ± 203.1 ± 0.2876 (4)
sa223/qC125.5°73 ± 126 ± 1 (100)1.3 ± 0.61561 (7)
sa223/qC122.5°80 ± 25 ± 0.8 (20)15 ± 4e,f816 (2)
sa223/qC120°85016e,g476 (1)
sa223/qC115°76 ± 3024 ± e,h493 (2)
m65/qC125.5°69 ± 222 ± 2 (88)9 ± 1753 (4)
m4125.5°097 ± 23 ± 21508
e97925.5°00100605
sa22325.5°095 ± 105 ± 1031
sa22322.5°03.3 ± 497 ± 4i102
sa22320°00100130
sa22315°0010029
  • a Estimated percentage of the homozygous daf-2 segregants displaying this phenotype shown in parentheses.

  • b Primarily unhatched eggs, L1s, or clear, unhealthy-looking L2s.

  • c Total number of progeny scored. Number of trials given in parentheses.

  • d Includes a small proportion of late L2ds.

  • e Probably underestimated due to scoring homozygous sa223 adults with extensive gonad development as wild type.

  • f 0.4 ± 0.2% dead eggs; 2 ± 0.6% L2d; 12 ± 4% dauer-like L3 and L4 forms, and adults without discernible gonad develpment; 1 ± 0.7% adults with some degree of gonad development (underestimated). Scored 80 hr after egg laying.

  • g 0.6% dead eggs; 12% dauer-like L3 and L4 forms, and adults without discernible gonad development; 3% adults with some degree of gonad develpment (underestimated). Scored 96 hr after egg laying.

  • h 0.5 ± 0.1% dead eggs; 4 ± 1% L2ds; 20 ± 1% dauer-like L3 and L4 forms. Scored 120 hr after egg laying.

  • i Developmental arrest at L2d stage.