TABLE 13

Dauer larva formation in daf-2 and daf-12(m20) strains

22.5°25.5°
60 hr80 hr50 hrTerminal phenotypea
AlleleAppearanceLarval arrest (%)Adult (%)NbAppearanceDauer arrest (%)Other larval arrestcAdults (%)Nb,d
N2L4, adult01003133
Class 1
daf-2(el365)L4, adult01002641L2d molt95.5 ± 0.54.5 ± 0.50897
daf-2(m577)L4, adult01002590L2d molt97.0 ± 1.03 ± 10816
daf-2 (sa193)L4, adult01002675Post-L2d molte99.5 ± 0.5f0.5 ± 0.50f704
daf-2(m41)Dauer, L472.727.32296L2d molt10000834
daf-2(m212)Dauer99.60.51738Post-L2d molte98.2 ± 0.21 ± 0.60.8 ± 0.4933
daf-2(el369)Post-L2d molte98.3 ± 0.71.7 ± 0.70224
daf-2(m41); daf-12(m20)L4, adult0100653L3, L400.5 ± 0.599.5 ± 0.5794
daf-2(el365); daf-12(m20)L4, adult0100536L3, L400.4 ± 0.199.6 ± 0.1739
daf-2(m577); daf-12(m20)L4, adult0100732L3, L402 ± 198 ± 1755
daf-2 (sal 9 3); daf-12(m20)L4, adult0100840L3, L400100589
daf-2(m212); daf-12(m20)L4, adult0100587L3, L400100801
daf-2(el369); daf-12(m20)L400.7 ± 0.199.4 ± 0.1316
Class 2
daf-2(m596)L2d molt95.1 ± 1.04.9 ± 1.00431
daf-2(el370)Dauer, L419.480.62318L2d molt91.6 ± 5.18.4 ± 5.101974g
daf-2(el391)Dauer10001353L2d molt89.5 ± 0.510.5 ± 0.50340
daf-2(e979)Dauer10001847Unhatched egg, L10100h0339
daf-2(m596); daf-12(m20)L401.5 ± 0.698.6 ± 0.6368
daf-2(el370); daf-12(m20)L3, L4100i070L1 – L2j0100k0284
daf-2(el391); daf-12(m20)L3, L4100i083L2 – L3l0100m0542
daf-2(e979); daf-12(m20)L3, L4100i0189Unhatched egg, L10100n0325
  • a Measured between 50 and 100 hr.

  • b Sample size.

  • c L1 arrest, except where indicated.

  • d Two simultaneous trials performed.

  • e Nascent dauer larvae undergoing radial shrinkage.

  • f 99.5 ± 0.5% dauer larvae at ~73 hr. By ~95 hr approximately 10% of the dauer larvae were recovering.

  • g Nine trials.

  • h 86.5 ± 1.5% unhatched eggs, 13.5 ± 1.5% L1 arrest.

  • i Animals remained developmentally arrested at a stage resembling L3 and L4 at 100 hr.

  • j L1s, L2ds, or larvae resembling intermediates between L2s and L2ds. Larvae later became Unc.

  • k 43 ± 0% L1 arrest, 57 ± 0% L2- and L3-sized arrest.

  • l Larvae resembled L2s or L3s in size, but were slightly darker, and later became Unc.

  • m 3 ± 0% L1 arrest, 97 ± 0% L2- and L3-sized arrest.

  • n 86 ± 2% unhatched eggs, 14.5 ± 1.5% L1 arrest, plus several L2s.