TABLE 4

Embryonic lethal phenotypes: Penetrance and parental tests

MutationP [m/m]aViable embryos (%) (n)R [m/m × +/+]bViable embryos (%) (n)S [m/+]cViable embryos (%) (n)
N297.3 (980)NAdNA
stu-8(oj1)62.2 (24l)70.6 (357)93.1(404)
abc-1(oj2)0.0 (277)0.0 (352)96.3(734)
spd-1(oj5)0.4 (820)1.7 (462)97.1(513)
zyg-1(oj7)0.0 (63l)0.0 (606)89.6(632)
stu-9(oj13)36.6 (298)24.3 (325)97.2(797)
stu-10(oj14)55.8 (727)32.6 (436)96.8(888)
stu-11(oj18)45.8 (714)51.7 (354)96.8(524)
stu-12(oj21)37.5 (267)42.9 (268)94.8(688)
slo-1(oj23)0.7 (305)30.5 (430)e95.5(797)
stu-13(oj24)4.1 (339)44.6 (547)e94.2(466)
stu-14(oj26)75.5 (347)75.3 (364)97.9(334)
stu-15(oj28)0.0 (261)52.2 (288)97.7(963)
spd-2(oj29)0.0 (255)0.0 (264)94.7(732)
stu-16(oj30)31.8 (42l)f96.1 (337)e97.0(405)
stu-17(oj31)74.6 (315)86.3 (526)97.5(868)
stu-18(oj32)11.9 (404)53.1 (386)e97.8(558)
stu-19(oj33)0.0 (503)0.0 (258)97.9(707)
cyk-2(oj34)20.6 (315)39.3 (267)e99.1(352)
spd-3(oj35)0.0 (464)0.0 (342)99.3(738)
  • a The percentage of viable embryos produced at 25° by homozygous mutant hermaphrodites. The number of embryos scored is indicated in parentheses. All animals were exposed to the restrictive temperature at the L4 larval stage except for stu-9 animals, which were exposed as young adults.

  • b The percentage of viable embryos produced at 25° by homozygous mutant hermaphrodites mated to wild-type males. The number of embryos scored is indicated in parentheses. All hermaphrodites were exposed to the restrictive temperature at the L4 larval stage, except for stu-9 animals, which were exposed as young adults.

  • c The percentage of viable embryos produced by heterozygous hermaphrodites at 25°. The number of embryos scored is indicated in parentheses. All animals were exposed to the restrictive temperature at the L4 larval stage.

  • d NA, not applicable.

  • e R-test value is significantly greater than corresponding P-test value (P > 0.05 by Student's t-test).

  • f A significant number of the animals that escaped embryonic lethality arrested at the L1 stage. In these tests, only 0.5% of the stu-16 animals and 0.7% of stu-18 animals were viable beyond the L1 stage.