TABLE 2

UV light-induced IS10 transposition with the acceptor plasmid pZF42

Mutation frequency × 107
StrainTransposition donorPoint mutationIS10 transposition
TK702(pZF42; pMVIS10)
0 J m−2pMVIS103.0 (1)10 (1)
30 J m−2pMVIS1085 (28)90 (9)
AB1157(pZF42; pMVIS10)
0 J m−2pMVIS100.16 (1)0.16 (1)
20 J m−2pMVIS1027 (169)2.4 (15)
30 J m−2pMVIS1053 (331)4.4 (27)
RKZ2(pZF42)
0 J m−2Chromosome0.11 (1)0.12 (1)
20 J m−2Chromosome10.7 (97)1.4 (12)
30 J m−2Chromosome20.0 (182)3.4 (28)
  • Cells harboring plasmids pZF42 and pMVIS10 or pZF42 alone were UV irradiated and assayed for TetR mutagenesis. The frequency of plasmid-to-plasmid IS10 transposition was determined from the fraction of cointegrates among all TetR mutants, as revealed by Southern blot analysis. The frequency of chromosome-to-plasmid IS10 transpositions was determined by Southern blot analysis of chromosomal DNA as described in Figure 6. The values in parentheses represent mutation frequency values relative to unirradiated cells.