TABLE 2

Postembryonic phenotypes caused by Stu mutations

Larval arrestaAdult PhenotypebVulval morphologyc
MutationEarlyMidLateSterileFertileMaleNormalPv1Vu1Muv
stu-8(oj1)400470011720
abc-1(oj2)d901320012630
spd-1(oj5)6005712006800
zyg-1(oj7)500290002251
stu-9(oj13)101650038000
stu-10(oj14)10046010271900
stu-11(oj18)e000677024000
stu-12(oj21)e3001373124200
slo-1(oj23)2004614437000
stu-13(oj24)e100386123000
stu-14(oj26)400267029100
stu-15(oj28)100650024034
spd-2(oj29)3400250118140
stu-16(oj30)10138230351610
stu-17(oj31)00027111100220
stu-18(oj32)e9341924018001
stu-19(oj33)101420042000
cyk-2(oj34)300520027000
spd-3(oj35)200371000190
  • a Number of animals that arrested during the L1/L2 (early), L2/L3 (middle), or L3/L4 (late) larval stages.

  • b Number of animals that developed to adulthood and were determined to be sterile hermaphrodites, fertile hermaphrodites, or males. The total number of animals scored is the sum of the number of animals exhibiting larval arrest and the number of animals scored for adult fates.

  • c A subset of adult hermaphrodites were scored for vulval morphology. Shown are the number of animals with one of the following morphologies: normal, not appreciably dissimilar from wild type; Pvl, protuding vulva; Vul, vulva absent; Muv, multiple (typically abnormal) value.

  • d Test performed using abc-1 heterozygous parents. Offspring of abc-1(oj2)/ + parents were transferred at the three fold stage of embryogenesis to 25°. Of 144 animals, 102 were phenotypically wild type. The remainder, which arrested as larvae or developed into sterile adults as indicated in the table, were inferred to be abc-1 homozygotes. Penetrance of the sterile phenotype was confirmed in independent tests using the abc-1/nT1 strain, in which abc-1 homozygotes could be unambiguously identified.

  • e In additional tests, these homozygotes were expossed to the restrictive temperature earlier in development by allowing gravid mothers to lay eggs for 3.25 hr at 25°. Of the animals produced during this period, 132 of 133 stu-11 hermaphrodites, 35 of 84 stu-12 hermaphrodites, 58 of 77 stu-13 hermaphrodites, and 51 of 60 stu-18 hermaphrodites developed into sterile adults.