Phenotype and polymorphic restriction site data for the Delta region

Abdominal bristlesSternopleural bristles
LineWBTCWBTC
MFMFMFMFMFMFMFMF
220.4523.2518.9520.9025.6828.4517.1820.2824.1024.7519.4321.2020.0022.9318.5819.23
418.2019.3019.0521.0325.3825.6517.2820.0819.3019.7018.4519.6319.7822.2317.9819.10
518.1018.3518.8322.5024.3827.5017.0020.0521.5020.9519.1819.8019.9523.1318.3319.18
622.5525.9019.2522.9525.8530.6817.4521.0323.0521.9019.0319.7819.7523.3318.4319.10
819.9524.1019.1321.9525.0329.5817.3520.4823.7024.9519.4320.3021.1522.5818.4319.30
922.5025.3020.9025.4527.6833.0518.4521.5519.2520.4520.8021.4520.8523.2019.9819.78
1022.9527.0521.7024.2326.2030.8518.3521.3325.0025.5519.7320.4321.6823.6319.6019.45
1220.1020.00**25.6328.8016.7320.2025.3024.70**20.9023.0018.4018.95
1320.3021.8517.9521.4025.0328.0517.4019.6519.3519.3018.0819.1019.1521.7318.1018.60
1419.4520.4519.2322.0025.3028.9317.5020.2026.7527.2519.1320.1320.5523.2019.1518.85
1521.4024.4519.3823.1525.4829.5317.6021.5022.6022.1520.0321.1821.1523.6318.7819.58
1621.0523.7520.1324.8026.3532.3317.9521.2520.9520.7518.7319.8019.7022.0518.5019.03
1720.6021.9520.1523.3326.9030.4817.8820.8322.9522.1020.5522.2022.2824.6519.3519.83
1920.1521.5517.2320.4324.5328.3316.3319.1323.0521.8019.1019.6319.7322.6018.0519.40
2219.3020.0519.3022.9324.9529.4317.3520.8020.6520.7518.3519.2019.6521.2018.5518.75
2320.7524.7518.4022.1025.4029.9017.5820.8021.1022.6518.1019.3819.4822.9318.1818.73
2420.0024.7018.5522.0524.1029.0517.4320.8324.9526.6019.0819.1820.2022.2019.3019.65
3118.2020.1018.9521.4825.0328.4517.9519.8316.1016.7017.0517.9518.8320.3017.2317.93
3220.3521.8519.8021.8824.9829.5817.2320.0820.0521.0018.4519.4020.1523.3018.4318.88
3317.4521.2018.7822.7324.6029.3017.1020.5318.9519.0018.5318.7519.6321.8818.0318.55
3420.8022.8017.9823.1324.8531.0817.0820.5319.4018.9518.0819.2819.9322.5318.3019.40
3619.3523.8020.3022.3326.1828.2517.9320.6820.7021.3518.5019.2320.2322.3318.7519.25
3820.5522.6019.1323.7324.0330.4317.1021.0019.6019.7018.8019.8820.2022.5818.4519.28
3920.5023.6519.0523.0025.2528.7517.5320.4318.1018.4017.9018.6319.8521.4017.8318.75
4118.3521.2518.6823.9523.7830.4516.6521.2821.7521.1518.7318.8019.3321.8018.0818.75
4421.4523.7519.8823.1524.7329.6517.0820.4318.9519.4019.9519.4519.7321.9518.9818.80
4519.7020.2519.3322.5825.6528.2517.7320.4318.3517.8518.3019.0020.3822.6318.2318.90
4619.1020.3517.6819.9524.4324.8817.0019.1815.8515.9518.7319.2319.6321.5318.2818.68
4817.9522.2018.8023.0524.5828.4017.0820.3821.9522.0519.3320.4020.4522.3817.9818.93
5018.5521.8518.4522.3324.0828.2516.9020.3322.1521.8020.2021.5021.2523.6819.7319.75
5119.2521.5519.0323.3025.1029.5316.5820.0018.7018.6517.8018.8018.9821.0817.9018.25
5321.0024.0018.8822.7325.7830.9817.4519.9518.8517.6518.1519.3319.7321.9818.5318.98
5522.2523.5020.4524.4524.6527.6016.8319.9822.0521.6519.6021.2020.8523.4818.6519.05
5819.7520.7518.4019.9325.2828.7017.2019.7524.4023.8517.3518.7819.4521.0818.0818.80
5922.4025.5020.2024.4525.8530.8517.6821.6323.2522.4518.3320.0320.5023.0819.0819.53
7416.6515.1021.0323.3325.2527.9817.6319.8023.2022.8519.6021.1820.7022.7317.9819.58
7521.0022.5019.7522.3824.8528.4016.6819.9821.7522.5019.1019.7820.6023.3818.3018.40
8120.4523.9018.5022.9824.4329.3016.9019.5524.2027.1518.9020.0819.9522.4318.7519.33
8420.8023.3019.3021.2326.2527.9817.6020.3323.1024.5520.1320.7520.8324.2318.3519.23
8520.4522.0018.8320.7325.6328.1817.2820.7519.2019.5518.3519.8020.0522.6017.8019.00
8621.8024.6019.2324.4324.5831.1817.9321.5519.3518.6518.3318.7520.0522.1318.3518.50
8721.4524.2519.6022.8525.5329.9517.9020.3821.8022.9019.3019.5320.0322.5518.4818.75
9419.6022.30**25.1828.6817.5319.5321.9022.30**21.3323.1519.2519.80
9521.2021.0016.6317.7523.9825.8815.8819.0020.6521.7519.1518.5819.7022.3017.8318.90
9722.1025.0021.8526.1324.4530.2818.9522.4022.0522.3518.4019.9819.8822.4818.0818.95
10126.6525.0521.6823.0326.0328.5818.6821.3523.6024.7519.7321.2021.3022.8319.0519.50
10518.6520.7518.0522.1323.1529.0516.7520.1021.1521.1017.9519.4519.5521.9818.3819.15
10622.5525.3520.1021.6024.9829.1317.6519.9023.6523.6519.4020.3021.1822.8318.8819.48
10720.3522.1021.4523.2026.2529.6318.1021.0818.4018.2518.3819.7819.8322.1817.8518.88
11118.7519.3017.8318.9524.0828.2016.7519.3521.0520.8018.8020.0519.8822.4518.4318.78
11317.5019.0018.3021.4325.7328.5016.7820.2523.6523.5519.2820.3020.3322.9018.4019.40
11515.8516.8518.5521.2025.3827.7517.8019.5321.9022.3018.0819.4319.6022.6818.5818.75
11619.8523.1521.8026.3026.2829.8317.9020.3321.6021.9021.3822.5321.5023.0018.5819.08
11720.9522.8019.0324.2825.5530.3017.9321.3322.0022.3518.1819.5020.5023.1318.0318.83
11921.6522.0019.7820.4825.3826.4017.1819.1522.4522.5519.4020.3820.7523.5518.9019.08
Sites
5′5′ reg
LineHi-24.7Hi-24.2P-23.9E-19.7E-17.2E-11.2P-9.1R-7.2B-4.8Ha-3.0A-2.9Ha-2.5C-2.4Ha-2.0Ha-1.9C-1.6Ha-1.3C-1.1C-0.7B+0.0Ha+1.0
2001010001100010000110
4000110011001011010010
5100110011101011011100
6101010011100110001110
8101110011000010000100
9001000011*00000000110
10101000011100010000010
12100010001100010000110
13001010011100110001100
14001000011100010000100
15001111010001000100011
16101110111011000001110
17111010011000010000010
19001010011100110001100
22101100001100010000010
23101110101100110001100
24001010011100110001110
31001000011000010000010
32001100011100000100011
33001110111001000001100
34101000011101001010110
36001011111001000000100
38101010011101100000000
39001010001001000001110
41101000011*00000000110
44100000001100000001110
45000010011100110001110
46101000011001000000010
48000010011101100000000
50001000011101100000000
51011000111111100000111
53001111010110000000101
55101100011000010000010
58101001101001000001110
59101100011001000001100
74101001111001000001100
75001110011000110001100
81001100011101111000010
84001000011100010000100
85001100011101111010010
86001000011101100000011
87001100001100010000010
94001111011100111011111
95101000011101100000000
97001000111100010000010
101001000011100011100111
105001000011101100000010
106001000001001011011100
107001010011100000000111
111001010011001001110011
113001000001001000001110
115001011010001000100011
116101100011101000000010
117001000011100010000010
119111110111100010001110
Sites
Intron 1Intron 2Intron 3Intron 53′ UTR
LineHa+3.5Ha+3.9A+4.2A+4.6A+4.9A+5.3C+5.6C+6.0Hi+6.2Hp+6.3Hp+6.7A+8.3Ha+8.6D+8.8A+9.4A+11.0C+11.7C+12.4C+13.1Hp+13.8Hp+14.5T+18.4S+18.6T+19.5A+19.6Ha+19.8R+19.9R+20.1T+20.4C+22.2T+23.4ΔC+2
211000000110011100001101010010000
411000000100011111000100010000010
510101010111011111010001011011000
611100000010011101001100010010110
811101011111011110000100011011010
911000010110****00001100011011000
1011010000100011111000100010011000
1211010100000011101001001010010000
1311000000010000110000101010010000
1401000110110001100001101010010110
1511100000110011110110101000000110
1601000110110****00001100011011000
1711000000010011100001101010010001
1911000000010000110000101010010000
2211000100110001111000101011011000
2311101001111011110000100010001110
2411100000010111101001101010010110
3111000010110000100001101010010110
3211100010110011110110101010000000
3301000110110001100001100011011000
3401000110110001100001101000010110
3611100000110000100011100010010001
3801000110110011111000100011011110
3911100000010011111000101011011000
4111010000110001100001100011011000
4411100000010011101001101011011010
4501000110110000100001101001010010
4610001001101000100001101011010000
4801000100110011111000100011011000
5001000110110011111000100011011000
5111010000010101000001010110101111
5311000000010101000001010110101110
5511000010110000100001101010010100
5811000100010010110000101010010000
5910001001111011100001101011011010
7410101001111011110010001011011000
7510100001111011100001111100000000
8111000000100011110000100000000**1
8410101010111011100001101000010110
8511000000100011110000100000000**0
8611000000110110100001000000011000
8711000000100000110000101010010000
9411000000110000100001000010100110
9501000101110000100001101010010100
9711100000010001110000101010000000
10111000000010011110000101011011010
10510001001101000110000100000000110
10610101001111011110010101011011010
10711000110110000101001100010010000
11111000000010011110000001010010000
11311000101110000100001101000010110
11511100000110011110110101010010000
11601000010110011100011100010010000
11711000000100011111000100010011000
11911000000100011101001101011011000
  • This section of the table gives mean abdominal and sternopleural bristle numbers in both males and females for each of the lines surveyed in each of the four genetic backgrounds studied. In the analyses of this paper the eight cells with missing data were replaced with the means for that sex/genetic background/bristle trait. The following sections give molecular marker genotype for markers in the Delta gene region; 1 indicates the presence of a site or band and 0 the absence of a site or band. The column heading “Location” gives the relative location in the Delta gene region, and the column heading “Site” gives the site name. The site name consists of a one- or two-letter designation indicating the restriction enzyme used to detect that site (A = AluI, B = BamHI, C = CfoI, D = Ddel, E = EcoRI, Ha = HaeIII, Hi = HndIII, Hp = HpaII, P = PstI, R = RsaI, S = ScrFI, T = TaqI) followed by a number indicating the exact position of the site in the Delta region (with some exceptions noted in Figure 1). Some of the markers had missing molecular marker data (in the case of those located in Intron 2, this was likely due to a polymorphism in the PCR priming site). In the case of missing molecular marker data, analyses were carried out with the lines with missing markers dropped for that particular marker. It is important to note that missing markers were permuted with the other markers, so the actual lines for which phenotypic measures were dropped varied over permutations.