Absence of Pac10p decreases the α-tubulin/β-tubulin ratio in the cell

Strainα-Tubulinβ-TubulinRatio, α/β
Δpac10, YCp502.13.30.63
Δpac10, pPAC103.13.20.93
  • Cultures of wild-type cells and Δpac10 haploids containing the indicated plasmids were grown on glucose. Protein extracts, normalized to cell number, were analyzed for α-tubulin and β-tubulin levels by immunoblotting (see materials and methods). Values were normalized to a nontubulin band in the α-tubulin immunoblot.