TABLE 4

Genetic distances between the ton1 mutation and chromosome 2 and 3 markers

MarkerChromosome (position in cM)Recombination percentaGenetic distance to ton1 (cM)No. of plants scored
RA122 (~15)45.8 ± 5.8Unlinked72 group 1 + 2
17.4 ± 5.518.1 ± 6.346 group 1
AKT12 (~48)18.1 ± 4.518.9 ± 5.272 group 1 + 2
0.00.046 group 1
m4292 (72.6)18.1 ± 4.518.9 ± 5.272 group 1 + 2
0.00.046 group 1
GAPA3 (40.1)47.2 ± 5.8Unlinked72 group 1 + 2
47.8 ± 7.4Unlinked46 group 1
GL13 (44.7)17.9 ± 4.316.5 ± 4.872 group 1 + 2
15.2 ± 5.215.7 ± 5.846 group 1
cdc2b3 (~71)0.00.072 group 1 + 2
0.00.046 group 1
BGL13 (71.9)0.00.072 group 1 + 2
0.00.046 group 1
  • An ACL4 heterozygous plant (WS background) and a Columbia wild-type plant were crossed. Seventy-two seedlings homozygous for the ton1 mutation (group 1 + 2) were selected in the F2 population deriving from this cross. The chromosomal structure of these mutants was analyzed by PCR and 26 of them were found to carry one wild-type chromosome 2 (group 2 plants). CAPS markers were tested on all F2 plants. To estimate the genetic distances in this cross between the ton1 mutation and several markers from chromosome 2, and to compare these distances to those derived from the reference RI map (Lister and Dean 1997), we had to remove group 2 plants from the analysis. The genetic distances and standard deviation were calculated using the Kosambi mapping function (Koornneef and Stam 1992).

  • a Values are ±SD.