TABLE 2

Genetic analysis of T-DNA transmission

Male > C24aFemale < C24b%Rself
LineHygR:HygS%RcTEmaledHygR:HygS%RcTEfemaledExpectedeObservedf
1140:21040.066.7146:37628.038.856.851.7
25:1832.72.7129:16843.476.845.153.0
3244:28246.486.5*60:15428.039.061.151.9
4102:17437.058.6205:35636.557.660.051.5
579:9646.282.3*88:33820.726.056.450.5
6112:16340.768.769:14232.748.660.350.3
826:14215.518.3174:28438.061.347.351.0
936:15219.123.777:15633.049.445.741.8
  • a Transgenic lines were used as the pollen donor.

  • b C24 was the pollen donor.

  • c %R = percentage HygR seedlings in backcross progeny.

  • d TEmale and TEfemale = the transmission efficiency (%) of the T-DNA.

  • e Expected percentage HygR seedlings in self-progeny based on observed male and female transmission was calculated as %Rself = %Rfemale + %Sfemale × (%Rmale/100).

  • f Observed %Rself was from selfed T4 progeny seedlings.

  • * The data showed no significant difference from 100% or a 1:1 segregation ratio.