TABLE 1

Segregation of hygromycin resistance and T-DNA copy number for eight putative T-DNA-tagged gametophytic mutants

Selfed T3Selfed T4Number of T4 linesb
LineHygR:HygS%RaHygR:HygS%Ra1:13:1All HygRTotalT-DNA copy Numberc
1134:14747.73197:298851.735352
2119:9056.95224:462953.04511471
3132:15146.64731:438551.946461
4148:12454.43671:345751.5365412
5121:14246.01747:171150.0253281
6148:11356.72337:231350.334341
8140:14149.81048:100751.022221
989:14138.71773:246841.837373–5
  • a % R, percentage of HygR seedlings.

  • b The number of lines show segregation ratios of 1:1, 3:1, and 100% HygR progeny.

  • c T-DNA copy number was determined by Southern blot analysis of EcoRI-digested genomic DNA with gus and hpt probes.