TABLE 2

Temperature of paralysis for heterozygous or transgene-rescued shi lethals.

ClassLineEmbedded Image Embedded Image Embedded Image Phenotype
IshiEM1436–37°34–35°no survivorsdom.-neg.
IshiEM3837°no survivorsno survivorsdom.-neg.
IshiEM53wild typeno survivors33°?
IshiEM3336°33°no survivorsdom.-neg.
IshiEM5537–38°36°<28°dom.-neg.
IshiEM6540°39°30°dom.-neg.
IshiEM6637°33°NDdom.-neg.
IIshiEM18wild typewild type34°recessive
IIshiEM27wild typewild type36°recessive
IIshiEM35wild typewild type35.5°recessive
IIshiEM42wild typewild type34°recessive
IIshiEM44wild typewild type34°recessive
IIshiEM56wild typewild type35.5°recessive
IIshiEM59wild typewild type36°recessive
IIIshi1218Hwild typewild type26° (as shits1)recessive
  • Paralysis is defined as the condition in which the animal lies on its back with little movement of the wings and legs. The temperature of paralysis is assessed by the occurrence of paralysis within 2 min at a specific temperature. Wild-type flies (ORR) are incapacitated at 42°; however, this behavior cannot be confused with the typical features of shi paralysis, which is preceded by characteristic behavioral seizures and uncontrolled wing-beating.