TABLE 3

Frequency of genotypes for the act2-1 allele found in successive generations segregating from a single heterozygous plant

GenotypeObserved no. of plants (%)Expected no. of plants (%)Embedded Image
F1 generation
A1A119 (25.7)18.5 (25)0.014
A1A240 (54)37.0 (50)0.243
A2A215 (20.3)18.5 (25)0.662
Total74 (l00)74 (100)χ2 = 0.919b
freq(A1) = 0.53afreq(A2) = 0.47P = 0.63
F2 generation
A1A152 (51.5)37.875 (37.5)5.268
A1A221 (20.8)25.25 (25)0.715
A2A228 (27.7)37.875 (37.5)2.574
Total101 (100)101 (100)χ2 = 8.56
freq(A1) = 0.62freq(A2) = 0.38P = 0.014
F3 generation
A1A158 (60)42.875 (37.5)5.336
A1A213 (14.7)12.25 (12.5)0.0459
A2A227 (25.3)42.875 (37.5)5.878
Total98 (100)98 (100)χ2 = 11.26
freq(A1) = 0.66freq(A2) = 0.34P = 0.0036
  • a Allele frequency of the F1, F2, and F3 generations of the heterozygous ACT2/act2-1 (A1A2) plants grown under high density greenhouse conditions. A1 and A2 denote the wild-type and T-DNA mutant alleles, respectively.

  • b χ2 value for the F1 generation is not significant with two degrees of freedom: One would expect to get this much deviation by chance >50% of the time. χ2 values for the F2 and F3 generations are significant with two degrees of freedom: One would expect to get this much deviation by chance <2% and <0.4% of the time, respectively.