Genotype | Observed no. of plants (%) | Expected no. of plants (%) | ||
---|---|---|---|---|
F_{1} generation | ||||
A_{1}A_{1} | 19 (25.7) | 18.5 (25) | 0.014 | |
A_{1}A_{2} | 40 (54) | 37.0 (50) | 0.243 | |
A_{2}A_{2} | 15 (20.3) | 18.5 (25) | 0.662 | |
Total | 74 (l00) | 74 (100) | χ^{2} = 0.919^{b} | |
freq(A_{1}) = 0.53^{a} | freq(A_{2}) = 0.47 | P = 0.63 | ||
F_{2} generation | ||||
A_{1}A_{1} | 52 (51.5) | 37.875 (37.5) | 5.268 | |
A_{1}A_{2} | 21 (20.8) | 25.25 (25) | 0.715 | |
A_{2}A_{2} | 28 (27.7) | 37.875 (37.5) | 2.574 | |
Total | 101 (100) | 101 (100) | χ^{2} = 8.56 | |
freq(A_{1}) = 0.62 | freq(A_{2}) = 0.38 | P = 0.014 | ||
F_{3} generation | ||||
A_{1}A_{1} | 58 (60) | 42.875 (37.5) | 5.336 | |
A_{1}A_{2} | 13 (14.7) | 12.25 (12.5) | 0.0459 | |
A_{2}A_{2} | 27 (25.3) | 42.875 (37.5) | 5.878 | |
Total | 98 (100) | 98 (100) | χ^{2} = 11.26 | |
freq(A_{1}) = 0.66 | freq(A_{2}) = 0.34 | P = 0.0036 |
↵^{a} Allele frequency of the F_{1}, F_{2}, and F_{3} generations of the heterozygous ACT2/act2-1 (A_{1}A_{2}) plants grown under high density greenhouse conditions. A_{1} and A_{2} denote the wild-type and T-DNA mutant alleles, respectively.
↵^{b} χ^{2} value for the F_{1} generation is not significant with two degrees of freedom: One would expect to get this much deviation by chance >50% of the time. χ^{2} values for the F_{2} and F_{3} generations are significant with two degrees of freedom: One would expect to get this much deviation by chance <2% and <0.4% of the time, respectively.