TABLE 2

Frequency of genotypes segregating for mutant actin alleles in the F2 generation

GenotypeObserved No. of plants (%)Expected No. of plants (%)Embedded Image
act 2-1
A1A130 (38.96)28.875 (37.5)0.004
A1A230 (38.96)19.250 (25)6.003
A2A217 (22.1)28.875 (37.5)4.884
Total77 (100)77 (100)χ2 = 10.931b
freq(A1) = 0.58afreq(A2) = 0.42P = 4.1 × 10−3
act4-1
A1A155 (56.7)36.375 (37.5)9.537
A1A227 (27.8)24.25 (25)0.312
A2A215 (15.5)36.375 (37.5)12.561
Total97 (100)97 (100)χ2 = 22.409
freq(A1) = 0.71freq(A2) = 0.29P = 1.4 × 10−5
act7-1
A1A128 (38.4)27.375 (37.5)0.014
A1A240 (54.8)18.25 (25)25.921
A2A25 (6.8)27.375 (37.5)18.288
Total73 (100)73 (100)χ2 = 44.224
freq(A1) = 0.66freq(A2) = 0.34P = 2.5 × 10−10
  • a Allele frequencies found in a sampling of the F2 generation of the originally transformed lines containing the act2-1, act4-1, or act7-1 T-DNA insertion mutations. A1 and A2 denote the wild-type and mutant T-DNA actin alleles, respectively.

  • b χ2 values are highly significant with two degrees of freedom: one would expect to get this much deviation from the expected result by chance <1% of the time (P ⩽ 10−2).