TABLE 1

Oligonucleotides used to assay allele frequencies

Gene/allelesOligonucleotide nameaLocationSequence 5′–>3′
ACT2 or act2-1
AAc2-I485SIvs LCTT CCT CAA TCT CAT CTT CT
AAc2-ATGN30 to
AAc2-ATGA30Start codon 1GCC TCA GCC ATT TTT TAT GAG CTG CAA ACA
RB16843SRight border T-DNAGCTCAGGATCCGATTGTCGTTTCCCGCCTTb
ACT4 or act4-1
ACT4-240SCodon 240AGC TTC GAG CTT CCT GAT GGA Cb
ACT4-3′252A3′ UTRAGC TCC CGG GAA TCT CTT TTG AGT AAC AAA TAA ATb
LB102ALeft border T-DNAGAT GCA ATC GAT ATC AGC CAA TTT TAG ACb
ACT7 or act7-1
AAc7-I3SIvs 3TTC CGC CTC TTT AAA ACT TTC AGC TCC ATT TAT
AA7-3′A23′UTRTGA ACC AAG GAC CAA ATA TAA TAT G
ACT 327SEx3ATG AAR ATN AAR GTN GTN GCN CCN CCN GA
GKBLB114ALeft border T-DNAGCC GGG ATC CAG GGC GTG TGC CAG GTG CCC ACG GAA TAG
  • a Each oligonucleotide is named based on its location and orientation in the actin gene or T-DNA sequence (e.g., oligonucleotides within a protein coding sequence contain a codon number, within the T-DNA a nucleotide number, within actin flanking sequence a 5′ or 3′, within an intron an I, S for sense orientation, and A for antisense orientation). Ivs, interveaning sequence or intron; Ex, Exon; UTR, untranslated flanking region.

  • b McKinney et al. 1995.