TABLE 5

Conjugational inheritance in exonuclease mutants

Relative recombination frequencyRelative recombination frequencyRelative plasmid transfer
StrainDesignation×JC158n×JC11033n×JC11033n
BT199Exo+(1)13(1)6(1)4
STL2694ExoI0.42 ± 0.2090.28 ± 0.1861.2 ± 0.234
STL2347ExoVII0.91 ± 0.239NDND
STL2331RecJ-0.88 ± 0.36130.88 ± 0.3460.91 ± 0.114
STL2758ExoI ExoVII0.90 ± 0.357NDND
STL2729ExoI RecJ0.10 ± 0.07110.07 ± 0.0260.92 ± 0.144
STL2348ExoVII RecJ0.60 ± 0.239NDND
STL2700ExoI ExoVII RecJ0.28 ± 0.1070.20 ± 0.124ND
  • Matings were performed as described in materials and methods with designated recipient above and either JC158 or JC11033 as donor. Recombinants were selected by Thr+ Leu+ [Ser+ Smr] and plasmid transfer by Kmr[Ser+ Smr]. Inheritance frequencies are expressed relative to that determined for BT199, which was 1-8% for chromosomal markers and 1-6% for plasmid transfer. Standard deviations of the mean are given for both relative recombination frequencies and for relative plasmid transfer. ND, not determined; n, number of determinations of relative frequency.