TABLE 3

Ethylene production in wild-type and mutant Arabidopsis

WScin1cin2Col.cin3cin4
TreatmentaEthylene produced (pl.seedling−1.24 hr−1)b
Control21 ± 418 ± 312.5 ± 2418 ± 415 ± 513 ± 3
BA (10 μmol)151 ± 1836 ± 252 ± 969 ± 1128 ± 1138 ± 4
2,4-D (50 μmol)179 ± 36181 ± 44116 ± 1281 ± 1395 ± 11103 ± 15
CuSO4 (20 mM)256 ± 28236 ± 32110 ± 10144 ± 14146 ± 41164 ± 36
24-epibrassinolide (600 nM)178 ± 34128 ± 1296 ± 19141 ± 3677 ± 12221 ± 58
TissueEthylene produced (pl.mg.−1.hr−1)
Flowers36 ± 640 ± 211 ± 270 ± 2880 ± 1071 ± 20
Siliques124 ± 5124 ± 23118 ± 14202 ± 15192 ± 18136 ± 32
Leaves0.9 ± 0.41.0 ± 0.21.0 ± 0.31.0 ± 0.31.3 ± 0.12.2 ± 0.7
Wounded leaves8 ± 19 ± 29 ± 311 ± 210 ± 316 ± 6
Light-grown seedlingsEthylene produced (pl.seeding−1.3 days−1)
102 ± 2285 ± 987 ± 23100 ± 994 ± 18103 ± 21
  • a Refers to treatment of two-day-old etiolated seedlings. These concentrations of BA, 2,4-D, CuSO4 and 24-epibrassinolide were chosen because they correspond to the peak of ethylene production observed in dose-reponse analysis in etiolated seedlings (K. Woeste, J. P. Vogel and J. J. Kieber, unpublished observations).

  • b Ethylene measurements are ± SD, based on 3 or 4 replicas.