Ethylene production in wild-type and mutant Arabidopsis
| WS | cin1 | cin2 | Col. | cin3 | cin4 | |
|---|---|---|---|---|---|---|
| Treatmenta | Ethylene produced (pl.seedling−1.24 hr−1)b | |||||
| Control | 21 ± 4 | 18 ± 3 | 12.5 ± 24 | 18 ± 4 | 15 ± 5 | 13 ± 3 |
| BA (10 μmol) | 151 ± 18 | 36 ± 2 | 52 ± 9 | 69 ± 11 | 28 ± 11 | 38 ± 4 |
| 2,4-D (50 μmol) | 179 ± 36 | 181 ± 44 | 116 ± 12 | 81 ± 13 | 95 ± 11 | 103 ± 15 |
| CuSO4 (20 mM) | 256 ± 28 | 236 ± 32 | 110 ± 10 | 144 ± 14 | 146 ± 41 | 164 ± 36 |
| 24-epibrassinolide (600 nM) | 178 ± 34 | 128 ± 12 | 96 ± 19 | 141 ± 36 | 77 ± 12 | 221 ± 58 |
| Tissue | Ethylene produced (pl.mg.−1.hr−1) | |||||
| Flowers | 36 ± 6 | 40 ± 2 | 11 ± 2 | 70 ± 28 | 80 ± 10 | 71 ± 20 |
| Siliques | 124 ± 5 | 124 ± 23 | 118 ± 14 | 202 ± 15 | 192 ± 18 | 136 ± 32 |
| Leaves | 0.9 ± 0.4 | 1.0 ± 0.2 | 1.0 ± 0.3 | 1.0 ± 0.3 | 1.3 ± 0.1 | 2.2 ± 0.7 |
| Wounded leaves | 8 ± 1 | 9 ± 2 | 9 ± 3 | 11 ± 2 | 10 ± 3 | 16 ± 6 |
| Light-grown seedlings | Ethylene produced (pl.seeding−1.3 days−1) | |||||
| 102 ± 22 | 85 ± 9 | 87 ± 23 | 100 ± 9 | 94 ± 18 | 103 ± 21 | |
↵a Refers to treatment of two-day-old etiolated seedlings. These concentrations of BA, 2,4-D, CuSO4 and 24-epibrassinolide were chosen because they correspond to the peak of ethylene production observed in dose-reponse analysis in etiolated seedlings (K. Woeste, J. P. Vogel and J. J. Kieber, unpublished observations).
↵b Ethylene measurements are ± SD, based on 3 or 4 replicas.