TABLE 4

QTL affecting GY, GM, KW, PC, and PH detected in maize TC progenies of F3 lines from cross P1 × P2 in Experiment 2 (calibration, N = 107) and respective QTL effects estimated in Experiment 1 (validation, N = 344)

Experiment 2Experiment 1
Position on chromosome (cM)LOD scoreEmbedded Image a (%)α-effectb (Mg ha−1)Embedded Image a (%)α-effectb (Mg ha−1)
ChromosomeT1T2T1T2T1T2T1T2T1T2T1T2
Grain yield (GY)
204.311.3−0.312.0−0.20**
31482.94.10.290.7−0.16
6504.816.50.430.0−0.00
61482.69.3−0.330.10.05
7562.711.10.299.80.49**
Totalc0.99.24.132.10.291.350.711.60.160.74
Grain moisture (GM)(g kg−1)(g kg−1)
1666.616.3−6.214.0−7.8**
2985.618.56.79.77.1**
21803.87.8−4.00.31.1
31022.58.6−3.83.1−3.6**
41403.68.5−4.26.2−5.1**
51082.87.53.71.02.3
51523.69.0−4.50.2−1.0
8643.011.1−5.15.5−5.7**
81145.621.111.56.68.3**
91545.510.3−4.42.0−2.6
1090783.110.70.125.7−0.48.50.47.41.66.5**
Total4.020.317.457.99.153.96.231.39.243.4
Kernel weight (KW)(mg)(mg)
41343.08.4−7.61.5−3.3*
5303.07.4−7.33.4−4.9**
5110942.62.86.718.8−7.0−11.811.79.3−9.9**−8.8**
774743.14.37.415.7−6.5−9.77.58.6−7.1**−7.2**
838525.14.225.815.2−15.4−10.120.916.4−14.4**−10.1**
9924.312.49.08.37.3**
Total11.313.641.543.736.148.335.933.036.236.7
Experiment 2Experiment 1
Position on chromosome (cM)LOD scoreEmbedded Image a (%)α-effectb (g kg−1)Embedded Image a (%)α-effectb (g kg−1)
ChromosomeT1T2T1T2T1T2T1T2T1T2T1T2
Protein concentration (PC)
12583.312.4−3.27.5−2.0**
21181143.83.115.911.5−3.1−2.55.74.8−1.6**−1.8**
7722.99.0−2.03.9−1.5**
850502.57.710.024.9−2.4−5.12.20.2−0.9**−0.5
8644.712.23.30.00.0
Total8.010.131.634.88.612.915.39.34.53.8
Plant height (PH)(cm)(cm)
12382325.96.327.923.5−10.4−9.8..11.720.7−6.7**−9.3**
31183.37.83.71.01.7
4362.810.3−5.40.2−0.9
5566.016.6−5.14.2−2.9**
8643.33.7−3.0.0.012.2−0.0
Total12.17.343.626.524.712.816.120.812.29.3
  • *, ** Significant at P < 0.05 and P < 0.01, respectively.

  • ., .. QTL × E interaction significant at P < 0.05 and P < 0.01, respectively.

  • a Percent phenotypic variance explained by QTL. For details see materials and methods.

  • b Average effect of substituting allele P1 by allele P2 in the TC progenies.

  • c Estimates of total LOD score and total variance explained obtained from simultaneous fit of all putative QTL for the respective trait.