TABLE 3

QTL affecting GY, GM, KW, PC, and PH detected in maize TC progenies of F3 lines from cross P1 × P2 in Experiment 1 (calibration, N = 344) and respective QTL effects estimated in Experiment 2 (validation, N = 107)

Experiment 1Experiment 2
Position on chromosome (cM)LOD scoreEmbedded Image a (%)α-effectb (Mg ha−1)Embedded Image a (%)α-effectb (Mg ha−1)
ChromosomeT1T2T1T2T1T2T1T2T1T2T1T2
Grain yield (GY)
12683.22.3−0.193.30.24
2225.76.0−0.382.3−0.22
5403.84.6−0.450.1−0.07
5644.04.00.430.10.07
51405.05.50.300.00.02
7743.19.80.462.10.15
8545.98.6−0.40..1.4−0.15
8803.56.4−0.351.7−0.15
10703.77.90.384.20.26
Totalc26.711.930.814.82.530.8212.23.81.040.30
Grain moisture (GM)(g kg−1)(g kg−1)
14612.815.8−9.8..9.7−7.7**
1683.46.0−3.61.6−2.0
12402384.77.55.98.5−4.7..−6.3..1.93.5−2.4−3.6
2225.25.84.2..1.31.8
21025.16.25.7.15.18.3**
21301388.913.59.810.55.86.8.0.22.70.7−3.0
21804.26.5−4.21.7−1.9
31149.612.1−5.81.3−1.7
31744.46.14.40.1−0.5
41301287.22.79.64.3−5.9−4.47.10.3−4.4*−1.0
6703.05.1−3.6..2.8−2.3
7204.83.86.03.74.4..3.5..0.00.10.10.5
730322.62.62.82.4−3.2..−3.1..0.40.5−1.1−1.2
7743.94.53.46.43.7*
852524.54.214.813.4−6.8−7.0..0.63.0−1.1−1.7
810611412.66.417.28.39.6.7.32.817.23.510.3**
91383.63.9−3.67.14.3*
108412.111.67.4..19.28.6**
Total45.258.245.655.159.274.924.346.426.951.3
Experiment 2Experiment 1
Position on chromosome (cM)LOD scoreEmbedded Image a (%)α-effectb (mg)Embedded Image a (%)α-effectb (mg)
ChromosomeT1T2T1T2T1T2T1T2T1T2T1T2
Kernel weight (KW)
12462325.46.97.17.0−6.8−6.43.13.6−5.5−5.5
274669.43.011.22.4−7.9−3.29.20.0−8.8**−0.3
21221223.15.95.17.25.96.80.10.10.81.0
21481564.75.16.84.8−6.3−5.30.30.01.60.4
31003.63.6−3.70.0−0.3
550568.27.811.98.7−6.8−5.814.08.6−8.6**−6.8**
51321285.88.416.410.2−8.3−6.8..3.54.0−4.2−5.1*
6704.54.8−3.90.0−0.4
7586015.913.521.114.9−10.7..−9.26.514.5−5.6*−9.5**
8484822.27.325.517.0−11.0−8.9*24.28.913.3**−7.9**
905.38.1−5.30.0−0.5
91021202.93.45.613.85.1..8.71.96.73.47.0**
101121126.75.48.54.65.84.3..4.07.04.26.1**
Total73.754.263.752.583.869.147.739.857.049.9
Protein concentration (PC)(g kg−1)g kg−1
123.74.01.21.81.0
12382209.12.612.41.2−2.4−0.611.60.8−3.5**−0.6
21461365.22.83.85.3−1.3−1.6.4.06.0−1.9−1.9*
21801802.83.33.72.6−1.2−1.00.20.10.40.2
31104.71.70.72.2−1.1
31644.84.7−1.411.9−2.7**
31983.11.40.70.00.1
4004.83.05.74.2−1.3−1.21.90.0−1.1−0.0
538363.912.22.811.10.9..2.1..0.04.70.11.6*
678745.811.75.813.4−1.3−2.3.0.30.2−0.40.4
61301326.44.57.06.51.51.60.01.1−0.1−0.8
7625.110.4−2.1..8.8−2.2**
Total35.241.937.743.011.215.019.525.78.611.4
Experiment 1Experiment 2
Position on chromosome (cM)LOD scoreEmbedded Image a (%)α-effectb (cm)Embedded Image a (%)α-effectb (cm)
ChromosomeT1T2T1T2T1T2T1T2T1T2T1T2
Plant height (PH)
11421266.23.615.25.5−4.3..−3.8..1.72.6−1.8−3.5
11845.56.4−4.1..0.71.8
123623017.627.027.925.2−7.6..−8.1..24.719.1−8.9**−9.0**
2202.64.02.50.2−0.7
21361445.95.35.96.22.62.82.60.72.31.2
31121163.26.81.88.11.43.2..7.03.03.4*2.6
31541644.97.36.38.83.44.0..1.32.61.92.8
411811410.79.612.712.3−5.0−5.1..6.93.2−4.4*−3.2
572904.03.39.35.7−3.4−3.120.80.3−6.8**0.9
51303.13.1−2.41.52.2
51524.55.93.20.00.3
61541565.98.08.18.1−2.9−3.11.60.7−1.8−1.3
704.55.7−2.62.3−2.1
740503.03.12.012.42.24.66.01.14.7*1.5
7686.23.42.70.5−1.2
8224.34.3−2.32.3−2.3
8527.18.5−3.08.7−4.0**
81445.14.52.10.0−0.2
9568.58.25.43.85.0
91362.74.02.70.8−1.5
1082706.06.610.16.24.12.8..4.40.03.20.2
Total72.773.563.263.658.452.155.737.553.533.1
  • *, ** Significant at P < 0.05 and P < 0.01, respectively.

  • .,.. QTL × E interaction significant at P < 0.05 and P < 0.01, respectively.

  • a Percent phenotypic variance explained by QTL. For details see materials and methods.

  • b Average effect of substituting allele P1 by allele P2 in the TC progenies.

  • c Estimates of total LOD score and coefficient of determination obtained from a simultaneous fit of all putative QTL for the respective trait.