TABLE 2

Single base pair substitutions detected in mutator or antimutator strains

GeneMutational targetG-C→A-TA-T-→G-CTotalG-C→T-AG-C→C-GA-T→C-GA-T→T-ATotalReference
Wild type
URA31616163243284Lee et al. 1988
URA326.73.33026.733.33.36.770von Borstel et al. 1993
SUP4-o25.514.54034.120.41.43.860Kang et al. 1994
SUP4-o25.315.540.833.419.52.34.059.2Roche et al. 1994
SUP4-o24.310.434.732.917.98.16.365.3Ramachandran 1996
SUP4-o32.38.74122.425.57.43.759Ramachandran 1996
CAN138.538.52338.561.5Tishkoff et al. 1997
Proofreading/polymerase
POL3 (Y)SUP4-o15.410.325.720.51.36.446.174.3Ramachandran 1996
POL3 (I)SUP4-o24.61438.632.71.810.516.461.4Ramachandran 1996
POL3URA3 LR2045655201035Morrison and Sugino 1994
POL3URA3 RL19.09.518.5439.51961.5Morrison and Sugino 1994
POL2URA3 LR23.323.3103.363.476.7Morrison and Sugino 1994
POL2URA3 RL10.051525204085Morrison and Sugino 1994
MUT7URA3 (30°)5.95.911.841.211.829.394.1von Borstel et al. 1993
MUT7URA3 (32°)6.76.713.333.346.793.3von Borstel et al. 1993
MUT7URA3 (34°)13.64.518.140.918.222.881.9von Borstel et al. 1993
Mismatch correction
PMS1 (Y)SUP4-o28.27.335.544.412.943.264.5Ramachandran 1996
PMS1 (I)SUP4-o27.07.534.642.115.834.565.4Ramachandran 1996
PMS1SUP4-o24,67.932.54615.14.81.667.5Yang 1995
MLH1SUP4-o17.26.924.144.810.420.775.9B.A. Kunz (unpublished data)
MSH2SUP4-o14.713.227.958.86.73.72.972.1Yang 1995
MSH2CAN16733100Marsischky et al. 1996
MSH3MSH6CAN114.228.642.857.257.2Marsischky et al. 1996
MSH6CAN155.611.166.727.85.533.3Marsischky et al. 1996
Nucleotide excision repair
RAD1SUP4-o24.76.631.334.126.63.34.768.7Kunz et al. 1990
RAD3-1SUP4-o23.21134.225.830.54.25.365.8Yang et al. 1996
RAD3-102SUP4-o10.82.413.257.825.31.22.486.8Montelone et al. 1992
REV3RAD1SUP4-o30.119.249.335.612.32.10.750.7Roche et al. 1994
RAD6-dependent repair
RAD6SUP4-027.510.638.159.82.161.9Kang et al. 1992
RAD18SUP4-011.1617.176.43.5382.9Kunz et al. 1991
REV3SUP4-023.820.444.240.810.90.73.455.8Roche et al. 1994
REV3RAD6SUP4-014.70.715.481.22.70.784.6Roche et al. 1995a
REV3RAD18SUP4-06.45.812.284.62.11.187.8Roche et al. 1995a
Recombinational repair
RAD52SUP4-026.55.832.323.537.62.24.467.7Kunz et al. 1989
REV3RAD52SUP4-026.111.537.644.87.31.88.562.4Roche et al. 1995a
Base excision repair
APN1SUP4-011.59.721.218.86.720.632.778.8Kunz et al. 1994a
APN1MAG1SUP4-019.710.630.313.66.124.225.869.7Kunz et al. 1991
UNG1SUP4-094.294.22.92.95.8Impellizzeri et al. 1991
Nucleotide metabolism
DCD1SUP4-o13.113.726.85.558.55.53.873.2Kohalmi et al. 1991
  • The values shown for each type of substitution are percentages of the total substitutions detected. The mutation rate for each type of substitution can be calculated by multiplying the relevant substitution rate determined from Table 1 by the percentage for each substitution. Other details are as described in the legend to Table 1.