TABLE 7

Frameshift revertants of the frameshift mutant his1-434(AAG) (his1-19)

Codon and peptidea sequences (5′-3′)Base changesNature of mutation (occurrences)Growth rateb (%)
GTTGAAAAAATGACCWild type
valglulysmetthr
GTTGAAAAGTGACCA−AA+Ghisl-434 (delAA → G)100
valglulysSTOP(his119)
GTTGAAAAAGTGACC+Arevertants (9)100
valglulysvalthr
GTTGGAAAAGTGACC+Grevertant (1)100
valglylysvalthr
GTTCAAAAAGTGACCG → C;+Arevertant (1)100
valglnlysvalthr
GTTAAAAAAGTGACCG → A;+Arevertants (2)100
vallyslysvalthr
GTTCAAAAATTGACCG → C;G → T;+Arevertants (2)100
valglnlysleuthr
GCTGAAAAAGAGACCT → C;T → A;+Arevertant (1)100
alaglulysgluthr
GGAAAAGTGACC−TTrevertant (1)100
glylysvalthr
GTTGAAATGACC−AGrevertant (1)~10
valglumetthr
GTTGAAAAAACCG → A;—TGrevertant (1)~33
valglulysthr
  • a The base number of the first base of the valine codon is 426.

  • b Percent of growth on −histidine medium compared with +histidine.