TABLE 5

Revertants of his1-798 (GA) (his1-7)

Mutant base numberBase changeAmino acid alterationMutant base numberBase changeAmino acid alteration
NonfeedersFeeders
15AAC → AAGasn5 → lys191ACT → AAThr64 → asn
23ACC → ATCthr8 → ile205GGT → AGTgly69 → met
115TCT → GCTser39 → ala205GGT → TGTgly69 → cys
206GGT → GTTgly69 → val206GGT → GCTgly69 → ala
226ACT → GCTthr76 → ala206GGT → GAT(2)agly69 → asp
227ACT → AGTthr76 → ser251TCT → TATser84 → tyr
367GTT → ATT(2)aval123 → ile367GTT → TTTval123 → phe
455GTC → GGCval152 → gly473GCA → GTAala158 → val
472GCA → ACAala158 → thr476TCA → TTA(2)aser159 → leu
478TGT → CGT(2)acys160 → arg492ATT → ATGile164 → met
487GGA→ AGAgly163 → arg612AGC → AGGser204 → arg
656GTC → GCCval219 → ala758TCC → TAC(2)aser253 → tyr
664GCT → TCT(2)aala222 → ser766GAC → TACasp256 → tyr
731ACG → ATGthr244 → met792AGT → AGGser264 → arg
733CCT → TCTpro245 → ser796ATG → TTGmet266 → leu
798bATA → ATGile266 → met
Undefinedc
18CAT → CAAhis6 → gln
76GTT → CTTval26 → leu
154GTA → TTAval52 → leu
199–201ΔGGTΔ gly67
605CCA → GCApro202 → arg
664GCT → ACTala222 → thr
744AGA → AGCarg248 → ser
  • a The number in parentheses indicates the number of mutants found to have the same mutation.

  • b Back mutation (restoration to wild-type genotype).

  • c The undefined mutants were taken from liquid cultures and were not tested for feeding or nonfeeding capability.