TABLE 4

Mapping of shy loci

shy mutationMarkerRecombinant chromosomesDeduced map position
shy2-2PVV4 nga63 shy2-115/320 8/320 0/102aChr. 1, 5 cM below PVV4, 3 cM above ngaB3, close to shy2-1
shy2-3shy2-20/109aChr. 1, close to shy2-2
amp1-4ptbbChr. 3, position 81
shy-115GL119/92Chr. 3, 21 cM from GL1
shy-802BGL13/56Chr. 3, 5 cM from BGL1
pom1-14PVV4 NCC14/60 5/60Chr. 1, 8 cM below PVV4, 8 cM above NCC1
pom1-15PVV46/60
NCC15/60
bot1-5ADH18/300Chr. 1, 6 cM below ADH
shy3-1m323 m4296/76 56/840Chr. 2, 8 cM below m323, 5 cM below m429
shy4-2nga8 GA114/58 8/58Chr. 4, 24 cM below nga8, 14 cM above GA1
shyB-1PVV4 NCC111/60 0/52Chr. 1, close to NCC1
  • a For mapping semidominant shy2 alleles relative to each other, we crossed two shy2 mutants, crossed the resulting F1 double heterozygote with wild type, and looked for phenotypically wild-type segregants among the F1 progeny of the outcross to wild type. Also included in the shy2-2 × shy2-3 data are 1065 F2 progeny, among which we observed no wild-type plants. This is equivalent to observing √1065 = 33 chromosomes in this cross of dominant mutations.

  • b pt is an allele of amp in the Landsberg erecta background. amp1-4 failed to complement pt, and no wild-type plants were observed among several hundred F2 progeny. Chr., chromosome.