TABLE 3

Segregation of shy mutant phenotypes among F2 progeny of backcrosses

shy mutationTall progenyaShort progenyaχ2bP
shy2-234890.46>0.25
shy2-3521630.08>0.5
amp1-4146520.17>0.5
shy-115133480.22>0.5
shy-80250160.02>0.5
pom1-1447221.74>0.1
pom1-1534110.01>0.5
bot1-53651042.00>0.1
shy3-1451002.82>0.05
shy4-178230.27>0.5
shy4-23811360.47>0.25
shy5-196391.09>0.25
shy6-1104350.01>0.5
  • a Tall and short F2 progeny were distinguished by eye in populations grown in dim white light. For those mutations with quantitative effects (shy3-1, shy4-1, shy4-2, shy5-1, and shyB-1), we measured hypocotyl lengths of representative F2 seedlings and found that the two visible groups (tall and short) formed a frequency distribution with two peaks, with no seed-lins of intermediate hypocotyl length.

  • b χ2 values were calculated for the null hypothesis of 3 tall:1 short segregation, except for the semidominant or dominant mutations shy3-1, shy2-2, and shy2-3, for which the null hypothesis was 1 tall:3 short.