TABLE 2

Viable punt alleles are temperature-sensitive and exhibit defects in wing venation

punt/punt
MatingTemperaturepunt/balancerViabilityWing phenotypeP value
A. puntP1/MKRS × punt10/MKRS22°80 (60)a53 (40)a15 (28)a1c
B. punt62/MKRS × punt10/MKRS22°224 (66)117 (34)39 (33)0.20
C. punt51/MKRS × punt10/MKRS22°267 (68)128 (32)9 (7)<10−6
D. punt88/MKRS × punt10/MKRS22°224 (73)81 (27)1 (1)<10−6
E. punt135/MKRS × punt10/MKRS22°267 (73)100 (27)1 (1)<10−6
F. punt10/MKRS × punt10/MKRS25°24 (67)12 (33)01d
G. punt10/MKRS × punt10/MKRS18°67 (87)10 (13)00.0015
  • a The numbers in parentheses indicate the percentage of total progeny. Because the MKRS/MKRS progeny are not viable, the expected frequency for the balanced class is 67% and for the unbalanced class is 33%.

  • b The numbers in parentheses indicate the percentage of total punt/punt homozygotes exhibiting a defect in wing venation.

  • c The tested parameter was wing phenotype. Observed and expected values (72% wt: 28% mutant) were compared using chi square analysis. P values < 0.05 were considered significant.

  • d The tested parameter was viability. Observed and expected values (67% punt/balancer: 33% punt/punt) were compared using chi square analysis. P values < 0.05 were considered significant.