TABLE 1

RFLP polymorphisms of four Aedes aegypti populations from Trinidad and Tobago, measured by observed heterozygosity and the number of alleles

CurepeCouvaSan FernandoTobago
ChromosomeLocusnHobsFISanHobsFISnHobsFISnHobsFIS
1LF9050.122−0.03630.159−0.07650.2560.160*30.3910.244***
LF230b30.2500.600***30.0690.880***30.1290.774***30.2960.535***
LF19860.6550.09660.5040.100**60.7750.02970.738−0.120**
LF17860.7610.01260.849−0.075*60.851−0.100***60.828−0.120**
TY750.5030.160*50.5460.151*50.543−0.00150.6560.060*
Average over chromosome 15.50.5100.0595.00.5150.0255.50.6060.0225.30.6540.016
2ARC150.750−0.04650.7240.01550.702−0.03860.7250.010
LF13840.729−0.08340.714−0.201**40.492−0.07540.4870.145**
LF28290.838−0.00970.7950.02580.864−0.060*70.793−0.102*
LF9850.6420.09250.654−0.03560.682−0.07360.878−0.068*
LF250a40.6150.06030.596−0.03240.800−0.154*30.562−0.183***
LF250b30.644−0.00930.687−0.03830.662−0.238***30.557−0.160*
LF11550.514−0.03150.366−0.01650.566−0.10140.562−0.077
Average over chromosome 25.00.676−0.0034.60.648−0.0405.00.681−0.1054.70.652−0.062
3LF35260.5480.268**60.7270.113**60.4410.292***60.5500.263***
LF26140.4830.03540.279−0.06140.476−0.05830.5920.008
para40.4690.15240.5630.02450.5980.08160.5550.103
LF16860.6670.042**70.5160.145**70.6670.10960.582−0.043
MalI40.6270.103**40.6370.00930.6400.03440.5770.137*
Average over chromosome 34.80.5590.1195.00.5440.0465.00.5640.0925.00.5710.093
Average over all loci5.10.5980.0504.80.5820.0035.10.626−0.0124.90.6270.006
  • * P< 0.05, ** P< 0.01, ***P< 0.001. Hobs, observed heterozygosity; n, number of alleles.

  • a Significant FIS also indicates distortion from HWE. Positive FIS indicates heterozygosity deficit from HWE expectation; negative FIS indicates excess of heterozygosity.

  • b A large proportion of individuals show an apparent gene deletion at the LF230 locus, therefore, this locus was not used for chromosomal average heterozygosity calculation. The heterozygosity and FIS were based on the individuals without deletions. The percentage of individuals showing the gene deletion at the LF230 locus was 41.4 for the Curepe population, 58.6 for Couva, 53.7 for San Fernando, and 46.0 for Tobago.