TABLE 1

Transfection frequencies with individual recombination substrates

Expt.aPlasmidEnzymeFrequency ± (× 106)b
1pSV2neoEcoRI1180 ± 160
2pSV2neoXEcoRI1260 ± 170
3pSV2neoS(B)XEcoRI<0.03
4pSV2neoS(B)XClaI<0.03
5pneoAnNXNheI0.4 ± 0.1
6pneoAnN11XNheI0.5 ± 0.3
  • a All transfections performed in wild-type (K1c) cells.

  • b Transfection frequencies were calculated as the ratio of G418r colonies per cell electroporated. Values are averages ± SD for three determinations. From 0.1 to 0.5 μg of each DNA were used in transfections; all frequencies are normalized to 0.1 μg of DNA.