TABLE 1

Percentage and range of mitochondrial genotype content and CV in C57BL/6 and NZB/BINJ cytoplast- and karyoplast-derived reconstructed embryos during the first six-cell cycles of preimplantation development

Cell cycleDonor mtDNANumberMean percentageRangeMean CVRange
Cytoplast1C57BL/60710.65.8–15.0
NZB/BINJ1010.97.0–16.0
2C57BL/61112.012.2–27.282.011.2–139.9
NZB/BINJ1211.57.6–15.460.123.1–121.8
3C57BL/61316.14.6–40.696.32.9–141.4
NZB/BINJ1311.04.6–28.278.043.3–130.3
4C57BL/61214.15.9–24.6101.044.9–140.0
NZB/BINJ1211.44.6–19.284.316.3–125.5
6C57BL/61115.28.7–23.3
NZB/BINJ1310.76.7–15.0
Karyoplast1C57BL/61318.712.1–24.6
NZB/BINJ1014.011.7–15.7
2C57BL/61318.114.0–22.455.411.8–90.9
NZB/BINJ1313.711.8–17.319.74.8–55.8
3C57BL/61217.37.7–23.447.415.1–72.8
NZB/BINJ1213.411.1–16.121.55.4–30.0
4C57BL/61222.318.5–27.046.628.8–87.6
NZB/BINJ1215.511.6–17.517.25.2–45.1
6C57BL/61429.513.9–43.0
NZB/BINJ1516.512.9–20.1
Persistent lineagea1LP22522.619.6–23.8
2LP22622.019.3–23.85.10–10.5
3LP22622.420.4–23.55.11.3–14.7
4LP22421.721.0–22.29.98.0–11.1
6LP22523.523.1–23.8
  • a The NZB/BINJ mtDNA content in embryos obtained from a nonsegregating, persistent heteroplasmic mouse lineage (LP22) originated from a C57BL/6 karyoplast-derived founder female.