TABLE 5

daf-2 mutants have wild-type brood sizes and reduced lethality at 15°

GenotypeTemperature for assayTotal progenya% Progeny arrest/lethalLife span (days)a
+/+15360 ± 17.4 (8)0.417.0 ± 1.0 (21)
25256 ± 15.6 (14)08.9 ± 0.2 (170)
daf-2(e1370)15293 ± 16.7 (7)039.5 ± 1.6 (37)
2544 ± 7.3 (21)15.421.6 ± 2.5 (18)
daf-2(e1391)15243 ± 18.0b (8)0.545.3 ± 3.2 (32)
2532 ± 5.2 (13)10.533.8 ± 2.2 (18)
daf-2(e1370)15240 ± 19.4 (7)0.229.7 ± 1.5 (36)
Backcrossed2571 ± 7.0 (15)9.322.7 ± 2.0 (22)
  • For each worm, the total number of eggs laid were counted (including dauers, nondauers, arrested larvae, and dead eggs) and averaged to obtain the total progeny. The % arrest/dead eggs was measured and the total number of animals that arrested or died was divided by the total number of progeny, as in Hengartner et al. (1992).

  • a Values are mean ± SE (n).

  • b daf-2(e1391) forms 0.7% dauers at 15° (n = 14/1000).