TABLE 4

Decreased brood size and viability in long-lived daf-2 and age-1 mutants

GenotypeStage shifted to 25°Total progeny after upshifta% Progeny arrest/lethalLife span at 25° (days)a
+/+L4256 ± 15.6 (14)08.9 ± 0.2 (170)
Young adult280 ± 20.4 (8)09.1 ± 1.1 (41)
daf-2(e1370)L444 ± 7.3 (21)15.421.6 ± 2.5 (18)
Young adult67 ± 8.2 (8)8.918.3 ± 1.1 (34)
daf-2(e1391)L432 ± 5.2 (13)10.533.8 ± 2.2 (42)
Young adult50 ± 4.1 (8)2.535.1 ± 2.6 (37)
daf-2(e1370)L471 ± 7.0 (15)9.322.7 ± 2.0 (22)
backcrossedYoung adult88 ± 8.0 (8)6.921.7 ± 2.0 (21)
age-1(hx546)L4189 ± 6.2 (10)015.6 ± 0.7 (108)
age-1(mg44)L476 ± 7.3 (11)5.820.7 ± 0.2 (26)
  • For each worm, the total number of eggs laid were counted (including dauers, nondauers, arrested larvae, and dead eggs) and averaged to obtain the total progeny after upshift. The % arrest/dead eggs was measured, and the total number of animals that arrested or died was divided by the total number of progeny, as in Hengartner et al. (1992). Previously, it has been shown that age-1(mg44) and age-1(hx546) have a reduced brood size at 25° (Gottlieb and Ruvkun, unpublished observations; Friedman and Johnson 1988a,b). Many of the age-1(mg44) animals at 25° die from internal hatching of the progeny, as they are slightly egg-laying defective (Gottlieb and Ruvkun 1994; our unpublished observations). These animals were not included in this experiment. age-1(mg44)/age-1(mg44) animals from age-1(mg44)/mnC1 mothers were singled to plates and the total number of progeny produced counted. These age-1(mg44)/age-1(mg44) animals from age-1(mg44)/age-1(mg44) mothers all arrested as dauers.

  • a Values (total progeny and life span) are mean ± SE (n).