TABLE 3

Courtship song parameters from recordings made at a mild temperature

GenotypenIPI (msec)CPP (no. cycles)Amplitude (arbitrary units)IPF (Hz)
wild-type1336.5 ± 0.43.0 ± 0.012.0 ± 0.4267.3 ± 4.6
parats11639.5 ± 0.5**3.0 ± 0.012.7 ± 0.3271.8 ± 4.9
tipE/tipE536.4 ± 0.42.9 ± 0.18.3 ± 0.6**241.8 ± 10.4*
napts1/+434.8 ± 0.63.1 ± 0.011.2 ± 0.4251.0 ± 6.1
napts1/napts1939.0 ± 0.7*3.1 ± 0.111.1 ± 1.0250.3 ± 6.3
cspX1/+735.4 ± 0.43.3 ± 0.1*15.4 ± 0.5**244.3 ± 4.7*
seits1/seP1634.8 ± 0.43.1 ± 0.212.3 ± 0.8250.0 ± 8.0
slo1/+436.3 ± 0.63.1 ± 0.011.1 ± 0.6240.5 ± 4.5*
slo1/slo1746.4 ± 0.8**2.6 ± 0.1*5.5 ± 0.3**229.0 ± 3.0**
slo1/slo2845.4 ± 0.6**3.2 ± 0.18.4 ± 0.3**247.9 ± 2.6
slo2/slo21040.9 ± 0.8**3.5 ± 0.1**8.3 ± 0.4**247.1 ± 4.8*
slo2/+1236.1 ± 0.63.1 ± 0.111.5 ± 0.3244.3 ± 3.4**
  • The results of computer-analyzing digitized courtship-song recordings, performed at 25°, are enumerated. The usual song parameters—IPI, CPP, amplitude, and IPF—were determined for genotypes indicated. As revealed in Figure 1, amplitudes were measured on an arbitrary scale, whereas IPIs and IPFs were measured in milliseconds (msec) and Hertz (Hz), respectively. Analysis of variance indicated significant differences between genotypes in all four parameters (IPI: F[11, 89] = 35.13, P < 0.001; CPP: F[11, 89] = 8.31, P < 0.0001; amplitude: F[11,89] = 24.61, P < 0.0001; IPF: F[11, 89], = 5.71, P < 0.0001). Asterisks highlight genotypes that significantly differ from the wild-type control at 5% (*) and 1%(**) levels according to Dunnett's method (see materials and methods).