TABLE 2

Knock-out tests under extreme heating

GenotypenNo. knocked-out after 10 min
RC29/+502
cac/RC295049
nbAEE171/RC29502
nbAH18/RC29504
  • Complete loss of bodily function, occurring when the key mutants were exposed to more extreme heating than used in Table 1. Here, a knock-out assay was carried out by placing the chamber-containing plastic wheel (preloaded with 1-5-day-old flies from different genotypes) on a hot-plate at ~46° (see materials and methods). Eight flies (two of each genotype) were assayed in a given execution of these tests. The difference between genotypes in the proportion of knocked-out flies is highly significant (χ2 = 158.29, d.f. = 3, P< 0.0001).