TABLE 1

Loss of body control in heated mutants

Percentage of time fly on its back or side
AgeGenotypen1–15 min16–30 min
1 dayRC29/+100.9 ± 0.21.2 ± 0.4
cac/RC291023.1 ± 1.925.0 ± 3.5
9 daysRC29/+121.9 ± 0.43.4 ± 1.6
cac/RC291344.3 ± 2.357.9 ± 3.9
nbAEE171/RC2990.8 ± 0.22.9 ± 1.7
nbAH18/RC2981.4 ± 0.22.9 ± 0.9
20 daysRC29/+125.8 ± 0.915.6 ± 3.5
cac/RC291447.1 ± 3.377.6 ± 4.6
  • Convulsions exhibited by cacophony at 37° are compared among different genotypes involving this genetic locus and among adults of different ages. The proportions of time (in percentages) that these flies spent on their backs or side when exposed to the high temperature were measured, with the adult animals placed in chambers within a prewarmed plastic (matting-cell) wheel resting on a hot-plate (see materials and methods). Two to three flies were observed during a given (timed) observation. Wilcoxon/Kruskal-Wallis nonparametric tests showed that the differences between genotypes within each age class (e.g., cac/RC29 vs. the others for 9-day-old flies) and ages within each genotype at the two time intervals were significant in all cases. Significance levels were adjusted to 0.005, owing to experiment-wise error (cf. Sokal and Rohlf 1995).