TABLE 2

Phenotypes of meiotic mutants

StrainRelevant genotypePercent sporulationaPercent spore viabilitybUra prototrophs (X107)
MitoticcMeiototicd
JB128Wild type65.1968.6 ± 1.3 (1.0)720 ± 210 (1.0)
Y17red1-123327.2 ± 1.6 (0.8)390 ± 62 (0.5)
Y201mek1-104296.9 ± 1.5 (0.8)410 ± 100 (0.6)
YKR125spo14-3<0.1NA9.7 ± 1.7 (1.1)810 ± 206 (1.1)
Y195mum1-1/gsg1-24.5748.9 ± 0.9 (1.0)370 ± 61 (0.5)
JE626-19Bgsg1::LYS23.2757.6 ± 1.6 (0.9)230 ± 75 (0.3)
YKR137mum2-23.0NA6.7 ± 1.5 (0.8)470 ± 91 (0.7)
Y601mum2::LEU2<0.1NA4.8 ± 1.2 (0.5)240 ± 90 (0.3)
Y901mum2::LYS2<0.1NA5.0 ± 1.3 (0.6)220 ± 73 (0.3)
YKR150mum3-16.4385.9 ± 1.0 (0.7)410 ± 56 (0.6)
Y1002mum3::LEU2-7658.8339.3 ± 2.2 (1.1)600 ± 120 (0.8)
Y1078mum3::LYS27.2377.1 ± 0.8 (0.8)580 ± 61 (0.8)
YKR132mum4-127.0335.7 ± 1.1 (0.7)710 ± 148 (1.0)
  • a Percent sporulation was determined by phase-contrast microscopy; at least 300 cells were examined.

  • b Percent spore viability was determined by dissecting a minimum of 100 spores.

  • c Mitotic recombination frequencies ± SD represent the median frequency of nine independent cultures for strains JB128, YKR137, and Y901 and the median frequency for three independent cultures from strains Y195, JE626-19B, Y601, YKR150, Y1002, and YKR132. Numbers in parentheses are the frequency normalized to the frequency of the wild-type strain, JB128.

  • d The meiototic cultures were plated after 15 hr in sporulation medium and represent the mean value ± the SD from three independent cultures. JE626-19B, Y601, Y901, and Y1002 are transformants of JB128. The numbers in parentheses are the frequency normalized to the frequency of the wild-type strain, JB128. NA = not applicable.