TABLE 1

Redundancy of randomly selected 3532 ESTs revealed by RFLP analysis

ABProteinOrganismCDEFGHLoci
2194
341
426
58
6Methionine adenosyltransferase (EC 2.5.1.6)Acanthamoeba castellanii0501M3R2167, chr1, 58.4 cM: R2280, chr1, 65.9 cM:R476B, chr5, 20.9 cM
6Nonspecific lipid transfer protein Cw-21Hordeum vulgare0060M3S790C, chr11, 2.7 cM: S790A, chr11, 55.8 cM: S790B, chr12, 9.5 cM:
6HemoglobinH. vulgare5100M1C245, chr3, 30.5 cM:
6Aspartate aminotransferase, cytoplasmic isozyme 2 (EC 2.6.1.1)Arabidopsis thaliana4110M1C250, chr1, 132.4 cM:
6GOS2 proteinO. sativa3210M1C1467, chr7, 72.2 cM:
6Glutathione S-transferase IZ. mays0600M1R37, chr1, 132.4 cM:
7Phosphoglycerate kinase (EC 2.7.2.3), cytosolicTriticum aestivum5110M2R2403S, chr2, 17.5 cM: R2403, chr6, 106 cM:
7DNA-binding protein MNB1bZ. mays4210S1C607, chr6, 123.1 cM:
71141S1C854, chr1, 126.8 cM:
75-Methyltetrahydropteroyltriglutamate-homocysteine methyltransferase (EC 2.1.1.14)Haemophilus influenzae0421M1R1759, chr12, 108 cM:
8dTDP-glucose 4-6-dehydratases homologA. thaliana2600S1C614, chr3, 43.9 cM:
8Tubulin α-1 chainO. sativa4211M1C1468, chr3, 132 cM:
8Transmembrane proteinZ. mays0341M1R427, chr2, 107.7 cM:
8Thionin precursor, leafH. vulgare0800M1R1382, chr7, 49.6 cM:
9Histone H4 (TH091)T. aestivum8100M4C79, chr4, 97.6 cM: C2161, chr5, 92.8 cM: C1521, chr7, 75.7 cM: C2070, chr9, 61.5 cM:
9Ubiquitin precursor Ubi-1Z. mays2511M1R810, chr2, 15 cM
9ADP, ATP carrier protein precursorO. sativa3600M2C92, chr2, 123.1 cM: R2266B, chr6, 86.7 cM:
9Heat shock protien 82O. sativa5310M2R1562, chr9, 70.8 cM: C985, chr9, 84.7 cM:
9Leaf-specific thionin precursorH. vulgare0090M1S1809, chr6, 66.1 cM:
10Heat shock protein 70O. sativa3610M5C549, chr3, 88.7 cM: C1000, chr1, 143.5 cM: R2702B, chr2, 153.1 cM: R3182, chr5, 92.0 cM: S1524, chr3, 43.6 cM:
10Glyceraldehyde-3-phosphate dehydrogenase, cytosolicRanunculus acer6400M1C37, chr2, 90.4 cM:
14Chlorophyll a/b-binding protein II precursorO. sativa00014M1S10558, chr3, 88.7 cM:
17Alcohol dehydrogenase 1 (EC 1.1.1.1).O. sativa17000M1C496, chr11, 30.3 cM:
17Glyceraldehyde 3-phosphate dehydrogenase 2 (EC 1.2.1.12)Caenorhabditis briggsae71000S1R896, chr4, 74.8 cM:
18Fructose-bisphosphate-aldolase (EC 4.1.2.13), cytoslicO. sativa9711M3R2657A, chr1, 158 cM: R2657C, chr10, 9.75 cM: C2269S, chr5, 72.5 cM:
19Enolase 2 (EC 4.2.1.11)Z. mays71011M2C913A, chr10, 11 cM: R2185, chr3, 37.4 cM:
26Translation elongation factor eEF-1 α chainT. aestivum8981M1R518, chr3, 18.4 cM:
  • Column A indicates the number of redundancy of clones that show the same RFLP image by eight restriction enzymes. Column B shows the clone frequency with the redundancy less than six. The columns titled Protein and Organism show the name and organism of the most similar protein appearing in PIR database R48.0 and SWISS-PROT R33. Columns C–F show the redundancy in callus, root, etiolated shoot, and green shoot libraries, respectively. Column G represents the estimated number of gene copies by Southern analysis, and columns S and M represent the mean single copy and multiple copies, respectively. Column H shows the number of loci mapped by respective clone and name, and locations of the loci are presented in the column titled Loci.