TABLE 1

Plasmids used in this study

PlasmidDescription (protein designation)Origin
pRS313CEN HIS3 AmpRSikorski and Hieter (1989)
pRS314CEN TRP1 AmpRSikorski and Hieter (1989)
pRS315CEN LEU2 AmpRSikorski and Hieter (1989)
pRS316CEN URA3 AmpRSikorski and Hieter (1989)
pRS4242μ TRP1 AmpRChristianson et al. (1992)
pYW17pRS316SIS1 (WT)Yan and Craig (1999)
pYW62pRS314sis1Δ122–352 (ΔGMCTD)Yan and Craig (1999)
pYW65pRS314SIS1 (WT)Yan and Craig (1999)
pYW66pRS314sis1Δ173–352 (ΔCTD)Yan and Craig (1999)
pYW116pRS314sis1Δ72–121 (ΔGF)Yan and Craig (1999)
pYW118pRS314sis1H34Q (H34Q)Yan and Craig (1999)
pGCH1pRS314sis1K199A (K199A)This study
pAK1pRS314sis1Δ339–352 (ΔDD)This study
pJ528pRS315SUP35MCThis study
pMR26LpRS315PCUP1::Hsp104Reidy and Masison (2010)
pAK17pRS314sis1H34Q/Δ339–352 (H34Q/ΔDD)This study
pAK18pRS314sis1K199A/Δ339–352 (K199A/ΔDD)This study
pAK21pRS314sis1Δ122–172 (ΔGM)This study
pAK29pRS314sis1Δ122–352c-myc (ΔGMCTDc-myc)This study
pAK30pRS314sis1Δ173–352c-myc (ΔCTDc-myc)This study
pAK31pRS314sis1Δ339-352c-myc (ΔDDc-myc)This study
pAK32pRS314SIS1-c-myc (c-myc)This study
pAK33pRS315SUP45 (Sup45)This study
pAK50pRS314HDJ1 (Hdj1)This study
pAK64pRS314sis1H34Q/K199A (H34Q/K199A)This study
pRS313RNQ–GFPpRS313 (Rnq1–GFP)This study