TABLE 3

Phenotypes of hemizygous mutant strains

Percent animals that display mutant phenotypea
AlleleCAN positionbClrcWitUncEglMuvLmpc
cam-1(gm105)34 ± 34 (56)0 (77)9 (55)87 (53)35 (20)0 (53)7 (77)
cam-1(gm105)/mnDf1616 ± 23 (30)0 (69)15 (40)85 (40)35 (20)0 (40)4 (69)
cam-1(gm122)7 ± 3 (25)1 (70)20 (50)100 (50)5 (20)2 (50)0 (70)
cam1(gm122)/mnDf1612 ± 18 (27)8 (66)16 (61)100 (56)37 (19)0 (62)2 (66)
fam-2(gm94)d35 ± 12 (47)0 (44)3 (59)7 (60)60 (20)0 (59)32 (59)
fam-2(gm94)/sDf2380 ± 22 (26)0 (50)15 (13)93 (13)8 (13)0 (13)0 (50)
syc-2gm132)88 ± 19 (53)0 (56)0 (44)100 (44)0 (20)0 (44)0 (56)
syc-2(gm132)/mnDf189 ± 15 (22)0 (65)0 (31)100 (31)0 (31)0 (31)0 (65)
syc-3(gm135)55 ± 31 (38)0 (76)0 (55)29 (58)0 (20)0 (55)1 (76)
syc-3(gm135)/qDf377 ± 29 (32)0 (62)0 (57)95 (57)0 (20)0 (57)0 (620
  • a Percentage of animals displaying the mutant phenotype is followed in parentheses by the number of animals scored. See materials and methods for a description of how the different phenotypes were scored.

  • b Average distance CAN cell migrated expressed as a percentage of the distance traveled in wild-type animals (see materials and methods). CAN position in kyIs5 animals is 98 ± 5%. kyIs5 provides expression of GFP in the CAN cells.

  • c We were unable to distinguish homozygous mutant first stage larvae from hemizygous mutants. Therefore, these numbers include both.

  • d Two doses of kyIs5 increases the severity of CAN cell migration defects of Cam mutants and causes an Egl phenotype, whereas one dose of kyIs5 has no effect. fam-2 hemizygotes are probably less Cam and Egl than fam-2 homozygotes, because they have only one copy of kyIs5.