TABLE 2

Behavioral and morphological phenotypes displayed by Cam mutants

GenotypePercent ClrPercent WitPercent UncaPercent EglPercent MuvPercent Lmp
Wild type0 (80)0 (55)0 (55)0 (20)0 (55)0 (80)
kyIs50 (56)0 (55)0 (55)80 (20)0 (55)2 (56)
cam-1(gm105)0 (77)9 (55)87 (53)70 (20)0 (53)6 (77)
cam-1(gm122)38 (8l)20 (40)100 (38)35 (20)0 (32)0 (84)
cam-2(gm124)19 (83)18 (61)100 (61)40 (20)10 (61)1 (84)
ceh-10(gm58)b100 (54)NANANANA0 (54)
ceh-10(gm120)3 (72)23 (70)15 (68)10 (20)19 (53)0 (72)
ceh-10(gm127)0 (80)36 (80)34 (70)10 (20)16 (63)0 (80)
epi-1(gm57)c0 (79)12 (49)100 (49)0 (20)22 (49)47 (79)
epi-1(gm121)29 (90)8 (47)79 (47)20 (20)11 (47)47 (90)
epi-1(gm139)0 (29)40 (38)100 (51)0 (20)11 (26)100 (29)
epi-1(gm146)2 (56)8 (66)64 (75)0 (20)3 (38)71 (56)
fam-1(gm85)0 (36)15 (34)77 (35)15 (20)0 (29)83 (36)
fam-2(gm94)0 (44)3 (59)7 (60)60 (20)0 (59)32 (59)
ina-1(gm144)0 (47)18 (33)83 (36)30 )20)0 (29)30 (46)
mig-2(gm38)0 (85)0 (46)100 (46)35 (20)2 (46)3 (85)
mig-2(gm103)0 (71)21 (63)100 (63)55 (20)12 (63)1 (71)
syc-1(gm126)1 (79)0 (60)35 (60)55 (20)0 (60)4 (79)
syc-2(gm132)0 (56)0 (44)100 (44)0 (20)0 (44)0 (56)
syc-3(gm135)0 (76)0 (55)29 (58)0 (20)0 (55)1 (76)
unc-34(gm104)0 (72)4 (48)100 (48)0 (20)0 (48)1 (72)
unc-34(gm114)0 (59)0 (53)100 (53)0 (20)0 (58)5 (59)
unc-34(gm115)0 (89)0 (58)100 (58)0 (20)0 (58)10 (81)
unc-34(gm134)2 (63)0 (54)100 (54)0 (20)0 (54)3 (63)
unc-73(gm67)0 (39)18 (50)100 (50)85 (20)10 (50)0 (39)
unc-73(gm123)0 (28)20 (51)100 (51)75 (20)12 (51)0 (28)
vab-8(gm99)3 (85)90 (67)97 (67)15 (20)25 (67)3 (85)
vab-8(gm138)3 (77)85 (72)96 (72)10 (20)28 (72)0 (77)
  • Percentage of animals displaying the mutant phenotype is followed in parentheses by the number of animals scored. See materials and methods for a description of how the different phenotypes were scored. Most mutants were characterized in the absence of the kyIs5 reporter, except for epi-1(gm121, gm139, gm146) and fam-2(gm94) (see materials and methods). The presence of kyIs5 enhances the Wit phenotype of Cam mutants and causes an Egl phenotype.

  • a Severity of Unc ranged from animals that moved reasonably well, but with exaggerated sinusoidal body waves (e.g., syc-3(gm135)), to animals that were immobile (e.g., mig-2(gm103sd)).

  • b ceh-10 strong mutations result in larval lethality and therefore phenotypes exhibited at later stages could not be determined. Although only data for gm58 is presented, gm71, gm100, gm131 and gm133 behaved similarly to gm58.

  • c Because epi-1 mutants are sterile or partially sterile, the penetrance of the Egl defect is reduced.